NTUBST2023EN

About the Common Equipment Room

Our Common Equipment Room is located at Room 517 of the New Agricultural Chemistry Building. The room was originally owned by our Biochemistry Division; in 2008, it was recruited into the Technology Commons of the College of Life Science (TechComm) and established as the TCX-C Facility Core. Currently, a total of 19 instruments and equipment are provided to members of the College of Life Science.

 

2024 (Academic Year 112):

Faculty in charge: Shih-Chung Chang (張世宗老師) (shihchung@ntu.edu.tw, 3366-3740) 

Technical personnel in charge: Yu-Ling Liang (梁瑜玲小姐) (yulingliang@ntu.edu.tw, 3366-2274)

Instrument administrators: Chia-Chun Hsiao (蕭嘉鈞) and Yi-Hsiang Tseng (曾翊翔)

 

TCX-C Facility Core use application guidelines:

1. The operating guidelines and rules are established by the TCX-C Management Committee, and all users are required to obtain permits. Workshops are held regularly in August and September every year.

2. During operation, please follow the rules and heed the precautions specific for each instrument. For first-time users, please contact the technical personnel and make an appointment with the instrument administrator (see below for more information regarding online appointment reservation).

3. Make sure to log in, make a registration, and log out. Contact the administrator as soon as possible if issues emerge.

4. Maintain access control security; people without permits are strictly prohibited from registering under someone else’s name or entering without authorization.

5. Thoroughly read the management policies, guidelines, penalties, and the three-part application form for TCX-C usage (DOC、ODF、PDF). 

 

Instrument reservation/cancellation forms for new TCX-C users  

Appointment reservation policy: Reservations for the following week should be made by the current Thursday; after Thursday, reservations can only be made for the week after the next.

The time slots available to make appointments with the administrators include Tuesday 10:00 am~12:00 am and Thursday 10:00 am~12:00 am.

There is a maximum number of five users to make reservations per time slot. The user will be notified by email after the reservation is confirmed.

 

Current TCX-C instrument/equipment reservation status

 

If the platform was helpful to your scientific publications, please acknowledge the Technology Commons, College of Life Science, National Taiwan University. For details, see here.

 

About payments: Adhering to the school payment guidelines, the TCX-C Management Committee has set a usage fee for each instrument/equipment. Users should provide their own required consumables and share the maintenance fees.

 

	Service Number	Instrument	Fee Schedule	Administrator (2024)	Contact Number	Faculty in Charge
1	TCX-C15	Ultracentrifuge	200 NTD/ hour (up to1200 NTD/ 24 hours)	李佳蓉	3366-4441	張世宗
2	TCX-C25	Centrifuge (Avanti J-25)	100 NTD/ use	李佳蓉	3366-4441	張世宗
3	TCX-C26	Centrifuge (Avanti J-26)	100 NTD/ use	李佳蓉	3366-4441	張世宗
4	TCX-C33	SpeedVac Concentrator	50 NTD/ hour	李佳蓉	3366-4441	張世宗
5	TCX-C37	Constant System Cell Disruptor	100 NTD/ 30 min#	李佳蓉	3366-4441	張世宗
6	TCX-C38A	Sonicator 3000	50 NTD/ 30 min#	李佳蓉	3366-4441	張世宗
7	TCX-C38B	Sonicator up200	50 NTD/ 30 min#	李佳蓉	3366-4441	張世宗
8	TCX-C39	Homogenizer	250 NTD/ hour	李佳蓉	3366-4441	張世宗
9	TCX-C63	ELISA Microplate Reader	20 NTD/ use*	李佳蓉	3366-4441	張世宗
10	TCX-C68	Spectramax-i3x Multi-mode Microplate Reader	100 NTD/ use*	曾翊翔	3366-9884	黃楓婷
11	TCX-C69	NanoDrop Spectrophotometer	40 NTD/ use*	曾翊翔	3366-9884	黃楓婷
12	TCX-C84	FPLC	50 NTD/ hour	李佳蓉	3366-4441	張世宗
13	TCX-C86C	Two-dimensional Electrophoresis (2-DE)	500 NTD/ use	李佳蓉	3366-4441	張世宗
14	TCX-C89C	Real-time PCR System	100 NTD/ hour	曾翊翔	3366-9884	黃楓婷
15	TCX-C91	ELGA Pure Water System (Q)	20/L	曾翊翔	3366-9884	黃楓婷
16	TCX-C91	ELGA Pure Water System (RO)	10/L	曾翊翔	3366-9884	黃楓婷
17	TCX-C93	Ice Maker Machine	5 NTD / 3 L	曾翊翔	3366-9884	黃楓婷
18	TCX-C97	UVP-DNA Image SystemDNA	30 NTD/30 min#	曾翊翔	3366-9884	黃楓婷
19	TCX-C98	UVP ChemiDoc-It Imaging System (Western)	30 NTD/30 min#	李佳蓉	3366-4441	張世宗

*：An additional charge will be incurred for every 20 minutes beyond.  #：A minimum of 30 minutes will be calculated for each use.

Note: The fee for individuals outside of this institution will be double that of the Life Sciences Institute.

Seahorse XFe24 Extracellular Flux Analyzer

Instrument Description:

The Seahorse Bioenergetics Analyzer is used to immediately detect the rates of oxygen consumption and acid production during cellular metabolism. Without contacting or harming cells, it continuously monitors changes in cellular energy by detecting variations in the concentration of oxygen and hydrogen ions in the cellular environment. It is patented and currently the only instrument globally capable of synchronously detecting both aerobic and anaerobic respiration, covering overall energy metabolism. This system has been widely applied in translational medicine, cancer research, aging, drug screening, toxicology, immunology, metabolic diseases, cell quality monitoring, and various other fields.

Instrument Services:

1. Measurement of oxygen consumption rate (OCR) of cells, tissues, or mitochondria.
2. Determination of extracellular acidification rate (ECAR) of cells or tissues.
3. Can measure up to 24 samples in a single run (3-4 blank samples are recommended).

Instrument Location (For reservations, please email):

Room 107, Red Brick House, Laboratory of Fu-Jung Lin (Department of Biochemical Science and Technology, College of Life Science, National Taiwan University)

Instrument Administrator: Jiang Zhong Lin (江忠霖)                               Office: 02-3366-1835

Cell Phone: 0930-592-991                          Email: eddieody@gmail.com

Operating Hours:

• Users must sign in/out according to the reserved time slots. Each reservation is for a 4-hour period, and usage within 4 hours is counted as 4 hours; usage exceeding 4 hours is counted as 8 hours.
• Operating Hours: Monday to Friday, 09:30-13:30 & 13:30-17:30.

Instrument Usage Guidelines:

1. Users must prepare all necessary consumables for experiments.
2. An oven is provided for the temporary storage of cells and calibration plates.
3. The facility does not provide space for cell culture, drug addition, or sample handling. Please dilute drugs and replace culture media before use.
4. The facility does not provide experimental training or assistance with instrument setup.
5. Please inquire with the manufacturer for experimental kits.

 

Fee Schedule (Effective from 2020/02/01):

Service Item	On-Campus	Off-Campus        Academic       Institutions	Off-Campus   Other Institutions
Independent        Operation	250 NTD/ 4 hours	500 NTD/ 4 hours	750 NTD/ 4 hours
Collaborative        Operation	500 NTD/ 4 hours	1000 NTD/ 4 hours	1500 NTD/ 4 hours

• Failure to check in for reserved time without prior cancellation will result in charges for the reserved duration.
• Extension of usage time may be permitted with additional fees if agreed upon by the administrator, without affecting other users. Charges will be based on the extended duration (4 hours/unit).
• Starting experiments earlier are allowed if they do not interfere with other users' rights. However, charges will be based on the actual instrument usage time length.
• Users are responsible for fees unless experimental results are adversely affected due to instrument malfunction.
• The administrator will settle instrument usage fees within a specified period and issue an invoice for reimbursement.
• Those who have not reached one full time period will be counted as one time period.
• These management regulations will be implemented after being announced, and adjustments may be made based on actual usage.
  ** Please carefully evaluate the time required to complete experiments to determine the length of the reservation. **

 

Student Laboratories

The department's laboratories include Student Laboratory 1 (Room 108), Student Laboratory 2 (Room 110), Biochemistry Laboratory (Room 214), and Nutrition Chemistry Laboratory (Room 216).

 

Borrowing Regulations:

Except for regular semester or summer courses, please submit a borrowing application in advance and adhere to the following guidelines:

1. Eligibility: Limited to courses under the guidance of instructors.
2. Borrowing Procedure: Submit the borrowing application form to the Department Office during office hours one week prior to usage. The form will be reviewed and approved by the Department of Biochemical Science & Technology.
3. Usage Period: During regular working hours on weekdays. Special approval is required for other time slots.
4. Return Procedure: Fill out the return receipt form, ensure the cleanliness of the laboratory environment, and verify the condition of all equipment. The responsible instructor must confirm the completion of the return procedure.
5. Damage Compensation: Users must handle laboratory equipment with care. In the event of damage or loss, excluding consumables under normal use, the user is responsible for repair or compensation at market value. If any damage to the laboratory equipment is noticed before the start of the course, the Department of Biochemical Science & Technology should be notified immediately, otherwise, the user will be held liable for compensation.

 

Precautions:

1. Strictly adhere to the safety regulations and warning signs in the laboratory.
2. Ensure to check and switch off water, electricity, doors, windows, and air conditioning equipment before leaving.
3. In the event of a safety incident during the borrowing period, the borrowing team and supervising instructor are fully responsible.
4. Regular course usage takes precedence over borrowing applications.
5. The department reserves the right to reclaim usage rights and terminate borrowing in special circumstances.
6. For any other matters not covered herein, follow the regulations of the university and other relevant laws and regulations.