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Core faculty

Associate Professor Hsuan-Chen Wu


Assistant Professor Hsuan-Chen Wu

Hsuan-Chen Wu

Title Associate Professor 
Education Ph.D., Bioengineering, University Of Maryland, College Park, MD
Research Expertise Synthetic Biology, Biofabrication, Biotechnology
LAB Synthetic Biology & Biofabrication Lab. (AG-109A)
TEL +886-2-3366-4524
Personal webpage  


  • Synthetic Biology: Prokaryotic/eukaryotic cellular reprogramming, quorum sensing switch
  • Metabolic Engineering: Autonomous protein expression systems, RNAi, cellular pathway engineering   
  • Biofabrication: Bio-inspired assembly, bio-device interfaces, bio-nano technology
  • Biomaterials: Spider silk, stimuli-responsive natural materials, tissue engineering scaffolds

If you are interested please contact me:


  1. Chen, W.-C., Wang, R.-C., Yu, S.-K., Chen, J.-L., Kao, Y.-H., Wang, T.-Y., Chang, P.-Y., Sheu, H.-S., Chen, S.-C., Liu, W.-R., Yang, T., Wu, H.-C. (2023) Self-Healable Spider Dragline Silk Materials. Advanced Functional Materials, 2303571. (IF 19.92)
  2. Sabarikirishwaran P, Shen M-Y, Ramaraj R, Unpaprom Y, Wu H-C, Chu C-Y. (2023) Feasibility and optimizing assessments on biogas and biomethane productions from E. coli fermenter effluent. Biomass and Bioenergy, 173, 106783. (IF 5.77)
  3. Wu, S.-D., Chuang, W.-T., Ho, J.-C., Wu, H.-C., Hsu, S.-H. (2023) Self-healing of recombinant spider silk gel and coating. Polymers, 15 (8), 1855 (IF 4.97)
  4. Wan H-Y, Chen Y-T, Li G-T, Wu H-C, Huang T-C, Yang T-I. (2022) Electroactive aniline tetramer–spider silks with conductive and electrochromic functionality. RSC Advances, 12(34), 21946-21956. (IF 4.08)
  5. Hu P, Ly KL, Pham LP, Pottash AE, Sheridan K, Wu H-C, Tsao C-Y, Quan D, Bentley WE, Rubloff GW. (2022) Bacterial chemotaxis in static gradients quantified in a biopolymer membrane-integrated microfluidic platform. Lab on a Chip, 22(17), 3203-3216. (IF 6.35)
  6. Terrell, J. L., Tschirhart, T., Jahnke, J. P., Stephens, K., Liu, Y., Dong, H., Hurley, M. M., Pozo, M., McKay, R., Tsao, C.-Y., Wu, H.-C., Vora, G., Payne, G. F., Stratis-Cullum, D. N., Bentley, W. E. (2021). Bioelectronic control of a microbial community using surface-assembled electrogenetic cells to route signals. Nature Nanotechnology, 16, 688–697. (IF 40.52)
  7. Hong J-C, Fan H-C, Yang P-J, Lin D-W, Wu H-C, Huang H-C. (2021) Localized proteolysis for the construction of intracellular asymmetry in Escherichia coli. ACS Synthetic Biology,10(8), 1830-1836. (IF 5.25)
  8. Wu S-R, Chen J-L, Wu H-C. Biofabricating a silk scaffold as a functional microbial trap. (2020) ACS Biomaterials Science & Engineering, 6(12), 7041-7050. (IF 4.75)
  9. Wu H-C, Pandey A, Chang L-Y, Hsu C-Y, Yang TC-K, Tso I-M, Sheu H-S, Yang J-C. (2020) Hydrothermal effect on mechanical properties of Nephila pilipes spidroin. Polymers, 12(5), 1013. (IF 4.33)
  10. Huang C-S, Hsieh S-C, Teng N-C, Lee W-F, Negi P, Belem WF, Wu H-C, Yang J-C. (2020) A silk fibroin based hydration accelerator for root canal filling materials. Polymers, 12(4), 994. (IF 4.33)
  11. Chen C-Y, Huang SY, Wan H-Y, Chen Y-T, Yu S-K, Wu H-C, Yang T-I. (2020) Electrospun hydrophobic polyaniline/silk fibroin electrochromic nanofibers with low electrical resistance. Polymers, 12(9), 2102. (IF 4.33)
  12. Virgile C, Hauk P, Wu HC, Bentley WE. (2019) Plasmid‐encoded protein attenuates Escherichia coli swimming velocity and cell growth, not reprogrammed regulatory functions. Biotechnology Progress, 35(3), e2778. (IF 2.33)
  13. Wu H-C, Wu S-R, Yang TC-K, Yang J-C. (2018) A facile measurement for monitoring dragline silk dope concentration in Nephila pilipes upon spinning. Materials, 11(10), 1951. (IF 2.97)
  14. Virgile C, Hauk P, Wu H-C, Shang W, Tsao C-Y, Payne GF, Bentley WE. (2018) Engineering bacterial motility towards hydrogen-peroxide. PLoS One, 13(5), e0196999. (IF 2.78)
  15. Liu Y, Wu H-C, Bhokisham N, Li J, Hong K-L, Quan DN, Tsao C-Y, Bentley WE, Payne GF. (2018) Biofabricating functional soft matter using protein engineering to enable enzymatic assembly. Bioconjugate Chemistry, 29(6), 1809-1822. (IF 4.35)
  16. Wu HC, Quan DN, Tsao CY, Liu Y, Terrell JL, Luo X, Yang JC, Payne GF, Bentley WE. (2017) Conferring biological activity to native spider silk: A biofunctionalized protein‐based microfiber. Biotechnology and Bioengineering, 114(1), 83-95. (IF 3.95)
  17. Wu HC, Quan DN, Tsao CY, Liu Y, Terrell JL, Luo X, Yang JC, Payne GF, Bentley WE. Cover Image, Volume 114, Number 1, January 2017. Biotechnology and Bioengineering,114(1), i-i. (IF 3.95)
  18. Tschirhart T, Kim E, McKay R, Ueda H, Wu H-C, Pottash AE, Zargar A, Negrete A, Shiloach J, Payne GF. (2017) Electronic control of gene expression and cell behaviour in Escherichia coli through redox signalling. Nature Communications, 8(1), 14030. (IF 12.35)
  19. Shang W, Tsao C-Y, Luo X, Teodoro M, McKay R, Quan DN, Wu H-C, Payne GF, Bentley WE. (2017) A simple and reusable bilayer membrane-based microfluidic device for the study of gradient-mediated bacterial behaviors. Biomicrofluidics, 11(4), 044114. (IF 2.57)
  20. McKay R, Hauk P, Wu HC, Pottash AE, Shang W, Terrell J, Payne GF, Bentley WE. (2017) Controlling localization of Escherichia coli populations using a two‐part synthetic motility circuit: An accelerator and brake. Biotechnology and Bioengineering, 114(12), 2883-2895. (IF 3.95)
  21. Servinsky MD, Terrell JL, Tsao C-Y, Wu H-C, Quan DN, Zargar A, Allen PC, Byrd CM, Sund CJ, Bentley WE. (2016) Directed assembly of a bacterial quorum. The ISME Journal, 10(1), 158-169. (IF 9.66)
  22. Quan DN, Tsao C-Y, Wu H-C, Bentley WE. (2016) Quorum sensing desynchronization leads to bimodality and patterned behaviors. PLoS Computational Biology, 12(4), e1004781. (IF 4.54)
  23. Kuo Y-C, Wu H-C, Hoang D, Bentley WE, D’Souza WD, Raghavan SR. (2016) Colloidal properties of nanoerythrosomes derived from bovine red blood cells. Langmuir, 32(1), 171-179. (IF 3.99)
  24. Zargar A, Quan DN, Emamian M, Tsao CY, Wu H-C, Virgile CR, Bentley WE. (2015) Rational design of ‘controller cells’ to manipulate protein and phenotype expression. Metabolic engineering, 30, 61-68. (IF 8.20)
  25. Terrell JL, Wu H-C, Tsao C-Y, Barber NB, Servinsky MD, Payne GF, Bentley WE. (2015) Nano-guided cell networks as conveyors of molecular communication. Nature Communications, 6(1), 8500. (IF 11.33)
  26. Luo X, Tsao C-Y, Wu H-C, Quan DN, Payne GF, Rubloff GW, Bentley WE. (2015) Distal modulation of bacterial cell–cell signalling in a synthetic ecosystem using partitioned microfluidics. Lab on a Chip, 15(8), 1842-1851. (IF 6.11)
  27. Liu Y, Wu H-C, Chhuan M, Terrell JL, Tsao C-Y, Bentley WE, Payne GF. (2015) Functionalizing soft matter for molecular communication. ACS Biomaterials Science & Engineering, 1(5), 320-328. (IF 5.67)
  28. Kim YW, Subramanian S, Gerasopoulos K, Ben-Yoav H, Wu H-C, Quan D, Carter K, Meyer MT, Bentley WE, Ghodssi R. (2015) Effect of electrical energy on the efficacy of biofilm treatment using the bioelectric effect. npj Biofilms and Microbiomes, 1(1), 1-8. (IF 3.13)
  29. Shi X-W, Wu H-C, Payne GF, Bentley WE. Fibrous assemblies for antibody presentation, and multiplexed antigenic analysis using same: Google Patents.
  30. Luo X, Wu H-C, Betz J, Rubloff GW, Bentley WE. (2014) Air bubble-initiated biofabrication of freestanding, semi-permeable biopolymer membranes in PDMS microfluidics. Biochemical Engineering Journal, 89, 2-9. (IF 4.45)
  31. Kim E, Xiong Y, Cheng Y, Wu H-C, Liu Y, Morrow BH, Ben-Yoav H, Ghodssi R, Rubloff GW, Shen J. (2014) Chitosan to connect biology to electronics: Fabricating the bio-device interface and communicating across this interface. Polymers, 7(1), 1-46. (IF 3.68)
  32. Adams BL, Carter KK, Guo M, Wu H-C, Tsao C-Y, Sintim HO, Valdes JJ, Bentley WE. (2014) Evolved quorum sensing regulator, LsrR, for altered switching functions. ACS Synthetic Biology, 3(4), 210-219. (IF 4..98)
  33. Wu HC, Tsao CY, Quan DN, Cheng Y, Servinsky MD, Carter KK, Jee KJ, Terrell JL, Zargar A, Rubloff GW. (2013) Autonomous bacterial localization and gene expression based on nearby cell receptor density. Molecular Systems Biology, 9(1), 636. (IF 14.10)
  34. Wu H-C, Hebert CG, Hung C-W, Quan DN, Carter KK, Bentley WE. (2013) Tuning cell cycle of insect cells for enhanced protein production. Journal of Biotechnology, 168(1), 55-61. (IF 2.88)
  35. Payne GF, Kim E, Cheng Y, Wu H-C, Ghodssi R, Rubloff GW, Raghavan SR, Culver JN, Bentley WE. (2013) Accessing biology's toolbox for the mesoscale biofabrication of soft matter. Soft Matter, 9(26), 6019-6032. (IF 4.15)
  36. Betz JF, Cheng Y, Tsao C-Y, Zargar A, Wu H-C, Luo X, Payne GF, Bentley WE, Rubloff GW. (2013) Optically clear alginate hydrogels for spatially controlled cell entrapment and culture at microfluidic electrode surfaces. Lab on a Chip, 13(10), 1854-1858. (IF 5.75)
  37. Terrell JL, Gordonov T, Cheng Y, Wu HC, Sampey D, Luo X, Tsao CY, Ghodssi R, Rubloff GW, Payne GF. (2012) Integrated biofabrication for electro‐addressed in‐film bioprocessing. Biotechnology Journal, 7(3), 428-439. (IF 3.45)
  38. Luo X, Wu H-C, Tsao C-Y, Cheng Y, Betz J, Payne GF, Rubloff GW, Bentley WE. (2012) Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling. Biomaterials, 33(20), 5136-5143. (IF 7.60)
  39. Liu Y, Terrell JL, Tsao CY, Wu HC, Javvaji V, Kim E, Cheng Y, Wang Y, Ulijn RV, Raghavan SR, Rubloff, G. W., Bentley,W. E., Payne, G. F. (2012). Biofabricating Multifunctional Soft Matter with Enzymes and Stimuli‐Responsive Materials. Advanced Functional Materials, 22(14), 3004-3012. (IF 9.77)
  40. Kim YW, Sardari SE, Meyer MT, Iliadis AA, Wu HC, Bentley WE, Ghodssi R. (2012) An ALD aluminum oxide passivated Surface Acoustic Wave sensor for early biofilm detection. Sensors and Actuators B: Chemical,163(1), 136-145. (IF 3.54)
  41. Cheng Y, Tsao CY, Wu HC, Luo X, Terrell JL, Betz J, Payne GF, Bentley WE, Rubloff GW. (2012) Electroaddressing functionalized polysaccharides as model biofilms for interrogating cell signaling. Advanced Functional Materials, 22(3), 519-528. (IF 9.77)
  42. Liu Y, Cheng Y, Wu H-C, Kim E, Ulijn RV, Rubloff GW, Bentley WE, Payne GF. (2011) Electroaddressing agarose using fmoc-phenylalanine as a temporary scaffold. Langmuir, 27(12), 7380-7384. (IF 4.19)
  43. Cheng Y, Luo X, Tsao C-Y, Wu H-C, Betz J, Payne GF, Bentley WE, Rubloff GW. (2011) Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation. Lab on a Chip, 11(14), 2316-2318. (IF 5.70)
  44. Tsao C-Y, Hooshangi S, Wu H-C, Valdes JJ, Bentley WE. (2010) Autonomous induction of recombinant proteins by minimally rewiring native quorum sensing regulon of E. coli. Metabolic Engineering, 12(3), 291-297. (IF 6.86)
  45. Fernandes R, Roy V, Wu H-C, Bentley WE. (2010) Engineered biological nanofactories trigger quorum sensing response in targeted bacteria. Nature Nanotechnology, 5(3), 213-217. (IF 30.31)
  46. Wu HC, Shi XW, Tsao CY, Lewandowski AT, Fernandes R, Hung CW, DeShong P, Kobatake E, Valdes JJ, Payne GF. (2009) Biofabrication of antibodies and antigens via IgG‐binding domain engineered with activatable pentatyrosine pro‐tag. Biotechnology and Bioengineering, 103(2), 231-240. (IF 3.65)
  47. Shi XW, Liu Y, Lewandowski AT, Wu LQ, Wu HC, Ghodssi R, Rubloff GW, Bentley WE, Payne GF. (2008) Chitosan biotinylation and electrodeposition for selective protein assembly. Macromolecular Bioscience, 8(5), 451-457. (IF 3.30)
  48. Shi X-W, Wu H-C, Liu Y, Tsao C-Y, Wang K, Kobatake E, Bentley WE, Payne GF. (2008) Chitosan fibers: Versatile platform for nickel-mediated protein assembly. Biomacromolecules, 9(5), 1417-1423. (IF 5.37)
  49. Hung Y-J, Smolyaninov II, Davis CC, Wu H-C. (2006) Fluorescence enhancement by surface gratings. Optics Express, 14(22), 10825-10830. (IF 3.55)
  50. Tso I-M, Wu H-C, Hwang I-R. (2005) Giant wood spider Nephila pilipes alters silk protein in response to prey variation. Journal of Experimental Biology, 208(6), 1053-1061. (IF 3.24)
  51. Sheu H-S, Phyu KW, Jean Y-C, Chiang Y-P, Tso I-M, Wu H-C, Yang J-C, Ferng S-L. (2004) Lattice deformation and thermal stability of crystals in spider silk. International Journal of Biological Macromolecules, 34(5), 267-273. (IF 2.60)