NTUBST2019

• 美國 馬里蘭大學學院市分校  生物工程學所  博士 (2005/08 ~ 2012/05)
• 東海大學  生物學所  碩士 (2000/08 ~ 2002/06)
• 國立台灣大學  化學工程學系  學士 (1996/08 ~ 2000/06)

• 合成生物學：細胞工程、群聚感應系統
• 代謝工程：自動蛋白質表達系統、 RNA干擾素、代謝途徑調控
• 生物製造：生物自組裝系統、生物裝置介面 、 奈米生物技術
• 生物材料：蜘蛛絲、刺激響應生物材料、組織工程材料

歡迎有興趣與熱情的同學加入研究團隊，請直接與我聯繫：hcwu7@ntu.edu.tw

• 生化科技學系 副教授 (2021/08 ~ present)
• 生化科技學系 助理教授 (2015/08 ~ 2021/07)
• 馬里蘭大學生命科學及生物科技研究中心  博士後研究員 (2012/05 ~ 2015/06)

1. Hu P, Ly K, Sheridan K, Wu HC, Tsao CY, Quan D, Bentley WE, Rubloff GW, Sintim HO, Luo X. Bacterial chemotaxis in static gradients quantified in a biopolymer membrane-integrated microfluidic platform. Lab Chip 2022; 22(17):3203-3216. (SCI)

2. Wan HY, Chen YT, Li GT, Wu HC, Huang TC, Yang TI. Electroactive aniline tetramer-spider silks with conductive and electrochromic functionality. RSC Adv 2022; 12(34):21946-21956.(SCI)

3. Terrell JL, Tschirhart T, Jahnke JP, Stephens K, Liu Y, Dong H, Hurley MM, Pozo M, McKay R, Tsao CY, Wu HC, Vora G, Payne GF, Stratis-Cullum DN, Bentley WE. Bioelectronic control of a microbial community using surface-assembled electrogenetic cells to route signals. Nat Nanotechnol 2021; 16(6): 688-97.(SCI)

4. Hong JC, Fan HC, Yang PJ, Lin DW, Wu HC, Huang HC. Localized Proteolysis for the Construction of Intracellular Asymmetry in Escherichia coli. ACS Synth Biol 2021; 10(8): 1830-6.(SCI)

5. 王瑞祺, 陳政良,吳亘承. 生物工程於重組蜘蛛絲之發展. 化工會刊 2021, 68卷第1期: 17-24

6. Wu SR, Chen JL, Wu HC. Biofabricating a Silk Scaffold as a Functional Microbial Trap. ACS Biomater Sci Eng 2020; 6(12): 7041-50.(SCI)

7. Wu HC, Pandey A, Chang LY, Hsu CY, Yang TC, Tso IM, Sheu HS, Yang JC. Hydrothermal Effect on Mechanical Properties of Nephila pilipes Spidroin. Polymers (Basel) 2020; 12(5): 1013.(SCI)

8. Huang CS, Hsieh SC, Teng NC, Lee WF, Negi P, Belem WF, Wu HC, Yang JC. A Silk Fibroin Based Hydration Accelerator for Root Canal Filling Materials. Polymers (Basel) 2020; 12(4): 994.(SCI)

9. Chen CY, Huang SY, Wan HY, Chen YT, Yu SK, Wu HC, Yang TI. Electrospun Hydrophobic Polyaniline/Silk Fibroin Electrochromic Nanofibers with Low Electrical Resistance. Polymers (Basel) 2020; 12(9): 2102.(SCI)

10. Virgile C, Hauk P, Wu HC, Bentley WE. Plasmid-encoded protein attenuates Escherichia coli swimming velocity and cell growth, not reprogrammed regulatory functions. Biotechnol Prog 2019; 35(3): e2778.(SCI)

11. Wu HC, Wu SR, Yang TC, Yang JC. A Facile Measurement for Monitoring Dragline Silk Dope Concentration in Nephila pilipes upon Spinning. Materials (Basel) 2018; 11(10).(SCI)

12. Virgile C, Hauk P, Wu HC, Shang W, Tsao CY, Payne GF, Bentley WE. Engineering bacterial motility towards hydrogen-peroxide. PLoS ONE 2018; 13(5): e0196999.(SCI)

13. Liu Y, Wu HC, Bhokisham N, Li J, Hong KL, Quan DN, Tsao CY, Bentley WE, Payne GF. Biofabricating Functional Soft Matter Using Protein Engineering to Enable Enzymatic Assembly. Bioconjug Chem 2018; 29(6): 1809-22.(SCI)

14. Shang, W., Tsao, C. Y., Luo, X., Teodoro, M., McKay, R., Quan, D. N., Wu, H. C., Payne, G. F., Bentley, W. E. (2017) A simple and reusable bilayer membrane-based microfluidic device for the study of gradient-mediated bacterial behaviors. Biomicrofluidics,11, 044114. doi: 10.1063/1.4993438. [EI, SCI]

15. McKay, R., Hauk, P., Wu, H. C., Pottash, A. E., Shang, Wu., Terrell, T., Payne, G. F., Bentley, W. E. (2017) Controlling localization of Escherichia coli populations using a two-part synthetic motility circuit: An accelerator and brake. Biotechnology and Bioengineering, 114:2883-2895. doi: 10.1002/bit.26391. [SCI, EI]

16. Tschirhart, T., Kim, E., Mckay, R., Ueda, H., Wu, H. C., Pottash, A. E., Zargar, A., and Negrete, A., Shiloach, J., Payne, G. F., Bentley, W. E. (2017) Electronic control of gene expression and cell behaviour in Escherichia coli through redox signaling. Nature Communications, 8:14030.  doi:10.1038/ncomms14030 [SCI]

17. Wu, H. C., Quan, D. N., Tsao, C. Y., , Liu, Y., Terrell, J. L., Luo, X. L., Yang, J. C., Payne, G. F., Bentley, W. E. Conferring biological activity to native spider silk: a biofunctionalized protein-based microfiber. Biotechnology and Bioengineering, 114:83-95. [SCI, EI]

18. Quan, D. N., Tsao, C. Y., Wu, H. C., Bebtley, W. E. (2016) Quorum sensing desynchronization leads to bimodality and patterned behaviors. PLOS Computational Biology, 12(4): e1004781

19. Kuo, Y. C., Wu, H. C., Hoang, D., Bentley, W. E., D’Souza, W. D., Ragjavan, S. R. (2016) Colloidal properties of nanoerythrosomes derived from bovine red-blood-cells. Langmuir, 32(1), 171-179. [SCI]

20. Servinsky, M. D., Terrell, J. L., Tsao, C. Y., Wu, H. C., Quan, D. N., Zargar, A. Allen, P., Byrd, C. M., Sund, C. J., Bentley, W. E. (2016). Directed assembly of a bacterial quorum. ISME Jorunal, 10(1), 158-169. [SCI]

21. Terrell, J. L., Wu, H. C., Tsao, C. Y., Barber, N. B., Servinsky, M. D., Bentley, W. E. (2015). Nano-guided cell networks as conveyors of molecular communication. Nature Communications, 6:8500. doi: 10.1038/ncomms9500 [SCI]

22. Kim, Y. W., Subramanian, S., Gerasopoulos, K., Ben-Yoav, H., Wu, H. C., Quan, N. D., Carter, K., Meyer, M., Bentley, W. E., Ghodssi, R. (2015) Effect of electrical energy on the efficacy of biofilm treatment using the bioelectric effect. NPJ Biofilms and Microbiomes, 1: 15016. doi:10.1038/npjbiofilms.2015.16. 

23. Kim, E., Xiong, Y., Cheng, Y., Wu, H. C., Liu, Y., Morrow, B. H., Ben-Yoav, H., Ghodssi, R., Rubloff, G. W., Shen, J., Bentley, W. E., Shi, X., Payne, G. F. (2015). Chitosan to connect biology to electronics: Fabricating the bio-Device interface and communicating across this interface. Polymers, 7, 1-46. [SCI, EI]

24. Zargar, A., Quan, D. N., Emamian, M., Tsao, C. Y., Wu, H. C., Virgile, C. R., Bentley, W. E. (2015). Rational design of “controller cells” to manipulate protein and phenotype expression. Metabolic Engineering, 30, 61-68. [SCI,EI]

25. Liu, Y., Wu, H. C., Chhuan, M., Terrell, J. L., Tsao, C. Y., Bentley, W. E., Payne, G. F. (2015). Functionalizing soft matter for molecular communication. ACS Biomaterials Science and Engineering, 1(5), 320–328.

26. Luo, X. L., Tsao C. Y., Wu, H. C., Quan, D. N, Payne, G. F, Rubloff, G. W, Bentley, W. E. (2015). Distal modulation of bacterial cell-cell signalling in a synthetic ecosystem using partitioned microfluidics. Lab on a Chip, 15(8), 1842-1851. [SCI] 

27. Luo, X. L., Wu, H. C., Betz, J., Rubloff, G. W., Bentley, W. E. (2014). Air bubble-initiated biofabrication of freestanding, semi-permeable biopolymer membranes in PDMS microfluidics. Biochemical Engineering Journal, 85, 2-9.  [SCI, EI]

28. Wu, H. C., Tsao, C. Y., Quan, D. N., Cheng, Y., Servinskyd, M. D., Carter, K. K., Jee, J. J., Terrell, J. L., Zargar, A., Rubloff, G. W., Payne, G. F., Valdese, J. J., Bentley, W. E. (2013). Autonomous bacterial localization and gene expression based on nearby cell receptor density. Molecular Systems Biology, 22, 9:636. Featured article. [SCI]

29. Wu, H. C., Hebert, C. G., Hung, C. W., Quan, D. N., Carter, K. K., Bentley, W. E. (2013). Tuning cell cycle of insect cells for enhanced protein production. Journal of Biotechnology, 168(1):55-61. [SCI; EI]

30. Fernandes, R., Roy, V., Wu, H. C., Bentley, W. E. (2010). Engineered biological nanofactories trigger quorum sensing response in targeted bacteria. Nature Nanotechnology, 5(3), 213-217. [ SCI]

31. Luo, X. L., Wu, H. C., Tsao, C. Y., Cheng, Y., Betz, J., Payne, G. F., Rubloff, G. W., Bentley, W. E. (2012). Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling. Biomaterials, 33(20):5136-43. [SCI, EI]

32. Adams, B., Carter, K. K., Guo, M., Wu, H. C., Tsao, C. Y., Sintim, H., Valdes, J. J., Bentley, W. E. (2014). Evolved quorum sensing regulator, LsrR, for altered switching functions. ACS Synthetic Biology, 3 (4), 210–219  [SCI]

33. Betz, J.F., Cheng, Y., Tsao, C.Y., Zargar, A., Wu, H. C., Luo, X., Payne, G. F., Bentley, W. E., Rubloff, G. W. (2013). Optically clear alginate hydrogels for spatially controlled cell entrapment and culture at microfluidic electrode surfaces. Lab on a Chip, 13(10), 1854-1858. [ SCI]

34. Payne, G. F, Kim, E., Cheng, Y., Wu, H. C., Ghodssi, R., Rubloff, G. W., Raghavan, S. R., Culver, J. N., Bentley, W. E. (2013) Accessing biology's toolbox for the mesoscale biofabrication of soft matter. Soft Matter, 9, 6019-6032. [SCI, EI]

35. Cheng, Y., Tsao, C. Y., Wu, H. C., Luo, X. L., Terrell, J. L., Betz, J., Payne, G. F., Bentley, W. E., Rubloff, G. W. (2012). Electroaddressing functionalized polysaccharides as model biofilms for interrogating cell signaling. Advanced Functional Materials, 22(3), 519-528. [SCI, EI]

36. Liu, Y., Terrell, J. L., Tsao, C. Y., Wu, H. C., Javvaji, V., Kim, E., Cheng, Y., Wang, Y., Ulijn, R. V., Raghavan, S. R., Rubloff, G. W., Bentley,W. E., Payne, G. F. (2012). Biofabricating multifunctional soft matter with enzymes and stimuli-responsive materials. Advanced Functional Materials, 22(14), 3004-3012. [SCI, EI]

37. Kim, Y. W., Sardari, S. E., Meyer, M. T., Iliadis, A. A., Wu, H. C., Bentley, W. E, Ghodssi, R. (2012). An ALD aluminum oxide passivated surface acoustic wave sensor for early biofilm detection. Sensors and Actuators B-Chemical, 163(1), 135-145. [SCI, EI]

38. Terrell, J. L., Gordonov T., Cheng Y., Wu H. C., Sampey D., Luo X., Tsao C. Y., Ghodssi R., Rubloff G. W., Payne G. F., Bentley W. E. (2012). Integrated biofabrication for electro-addressed in-film bioprocessing. Biotechnology Journal, 7(3), 428-439. [SCI, EI]

39. Cheng, Y., Luo, X., Tsao, C. Y., Wu, H. C., Betz, J., Payne, G. F., Bentley, W. E., Rubloff, G. W. (2011). Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation. Lab on a Chip, 11(14), 2316-2318. [SCI]

40. Liu, Y., Cheng, Y., Wu, H. C., Kim, E., Ulijn, R. V., Rubloff, G. W., Payne, G. F. (2011). Electroaddressing agarose using Fmoc-phenylalanine as a temporary scaffold. Langmuir, 27(12), 7380-7384. [SCI, EI]

41. Tsao, C. Y., Hooshangi, S., Wu, H. C., Valdes, J. J., Bentley, W. E. (2010). Autonomous induction of recombinant proteins by minimally rewiring native quorum sensing regulon of E. coli. Metabolic Engineering, 12(3), 291-297. [SCI, EI]

42. Wu, H. C., Shi, X. W., Tsao, C. Y., Lewandowski, A. T., Fernandes, R., Hung, C. W., DeShong, P., Kobatake, E., Valdes, J. J., Bentley, W. E. (2009). Biofabrication of antibodies and antigens via IgG-binding domain engineered with activatable pentatyrosine pro-tag. Biotechnology and Bioengineering, 103(2), 231-240. [SCI, EI]

43. Shi, X. W., Liu, Y., Lewandowski, A. T., Wu, L. Q., Wu, H. C., Ghodssi, R., Payne, G. F. (2008). Chitosan biotinylation and electrodeposition for selective protein assembly. Macromolecular Bioscience, 8(5), 451-457. [SCI, EI]

44. Shi, X. W., Wu, H. C., Liu, Y., Tsao, C. Y., Wang, K., Kobatake, E., Payne, G. F. (2008). Chitosan fibers: versatile platform for nickel-mediated protein assembly. Biomacromolecules, 9(5), 1417-1423. [SCI, EI]

45. Hung, Y. J., Smolyaninov, II, Wu, H. C., Davis, C. C. (2006). Fluorescence enhancement by surface gratings. Optics Express, 14(22), 10825-10830. [SCI, EI]

46. Tso, I. M., Wu, H. C., Hwang, I. R. (2005). Giant wood spider Nephila pilipes alters silk protein in response to prey variation. Journal of Experimental Biology, 208(6), 1053-1061. [SCI]

47. Sheu, H. S., Phyu, K. W., Jean, Y. C., Chiang, Y. P., Tso, I. M., Wu, H. C., Ferng, S. L. (2004). Lattice deformation and thermal stability of crystals in spider silk. International Journal of Biological Macromolecules, 34(5), 325-331. [SCI]