ntubst2019EN

• Synthetic Biology: Prokaryotic/eukaryotic cellular reprogramming, quorum sensing switch
• Metabolic Engineering: Autonomous protein expression systems, RNAi, cellular pathway engineering   
• Biofabrication: Bio-inspired assembly, bio-device interfaces, bio-nano technology
• Biomaterials: Spider silk, stimuli-responsive natural materials, tissue engineering scaffolds

If you are interested please contact me: hcwu7@ntu.edu.tw

1. Virgile, C., Hauk, P., Wu, H. C., Bentley, W. E. (2019, Jan). Plasmid encoded‐protein attenuates Escherichia coli swimming velocity and cell growth, not reprogrammed regulatory functions. Biotechnology Progress, e2778. [SCI]
2. Wu, H. C., Wu, S. R., Yang, T. C. K., Yang, J. C. (2018) A Facile Measurement for Monitoring Dragline Silk Dope Concentration in Nephila pilipes upon Spinning. Materials. 11: 1951 [SCI]
3. Virgile, C., Hauk, P., Wu, H. C., Shang, W., Tsao, C. Y., Payne, G. F., Bentley, W. E. (2018) Engineering Bacterial Motility towards Hydrogen-Peroxide. PLoS One. 3(5):e0196999. doi: 10.1371/journal.pone.0196999. [SCI]
4. Liu Y., Wu, H. C., Bhokisham, N., Li, J., Hong, K. L., Quan, D. N., Tsao, C. Y., Bentley, W. E., Payne, G. F. (2018) Biofabricating Functional Soft Matter Using Protein Engineering to Enable Enzymatic Assembly. Bioconjugate Chemistry. doi: 10.1021/acs.bioconjchem.8b00197. [SCI]
5. Shang, W., Tsao, C. Y., Luo, X., Teodoro, M., McKay, R., Quan, D. N., Wu, H. C., Payne, G. F., Bentley, W. E. (2017) A simple and reusable bilayer membrane-based microfluidic device for the study of gradient-mediated bacterial behaviors. Biomicrofluidics,11, 044114. doi: 10.1063/1.4993438. [EI, SCI]
6. McKay, R., Hauk, P., Wu, H. C., Pottash, A. E., Shang, Wu., Terrell, T., Payne, G. F., Bentley, W. E. (2017) Controlling localization of Escherichia coli populations using a two-part synthetic motility circuit: An accelerator and brake. Biotechnology and Bioengineering, 114:2883-2895. doi: 10.1002/bit.26391. [SCI, EI]
7. Tschirhart, T., Kim, E., Mckay, R., Ueda, H., Wu, H. C., Pottash, A. E., Zargar, A., and Negrete, A., Shiloach, J., Payne, G. F., Bentley, W. E. (2017) Electronic control of gene expression and cell behaviour in Escherichia coli through redox signaling. Nature Communications, 8:14030.  doi:10.1038/ncomms14030 [SCI]
8. Wu, H. C., Quan, D. N., Tsao, C. Y., , Liu, Y., Terrell, J. L., Luo, X. L., Yang, J. C., Payne, G. F., Bentley, W. E. Conferring biological activity to native spider silk: a biofunctionalized protein-based microfiber. Biotechnology and Bioengineering, 114:83-95. [SCI, EI]
9. Quan, D. N., Tsao, C. Y., Wu, H. C., Bebtley, W. E. (2016) Quorum sensing desynchronization leads to bimodality and patterned behaviors. PLOS Computational Biology, 12(4): e1004781
10. Kuo, Y. C., Wu, H. C., Hoang, D., Bentley, W. E., D’Souza, W. D., Ragjavan, S. R. (2016) Colloidal properties of nanoerythrosomes derived from bovine red-blood-cells. Langmuir, 32(1), 171-179. [SCI]
11. Servinsky, M. D., Terrell, J. L., Tsao, C. Y., Wu, H. C., Quan, D. N., Zargar, A. Allen, P., Byrd, C. M., Sund, C. J., Bentley, W. E. (2016). Directed assembly of a bacterial quorum. ISME Jorunal, 10(1), 158-169. [SCI]
12. Terrell, J. L., Wu, H. C., Tsao, C. Y., Barber, N. B., Servinsky, M. D., Bentley, W. E. (2015). Nano-guided cell networks as conveyors of molecular communication. Nature Communications, 6:8500. doi: 10.1038/ncomms9500 [SCI]
13. Kim, Y. W., Subramanian, S., Gerasopoulos, K., Ben-Yoav, H., Wu, H. C., Quan, N. D., Carter, K., Meyer, M., Bentley, W. E., Ghodssi, R. (2015) Effect of electrical energy on the efficacy of biofilm treatment using the bioelectric effect. NPJ Biofilms and Microbiomes, 1: 15016. doi:10.1038/npjbiofilms.2015.16. 
14. Kim, E., Xiong, Y., Cheng, Y., Wu, H. C., Liu, Y., Morrow, B. H., Ben-Yoav, H., Ghodssi, R., Rubloff, G. W., Shen, J., Bentley, W. E., Shi, X., Payne, G. F. (2015). Chitosan to connect biology to electronics: Fabricating the bio-Device interface and communicating across this interface. Polymers, 7, 1-46. [SCI, EI]
15. Zargar, A., Quan, D. N., Emamian, M., Tsao, C. Y., Wu, H. C., Virgile, C. R., Bentley, W. E. (2015). Rational design of “controller cells” to manipulate protein and phenotype expression. Metabolic Engineering, 30, 61-68. [SCI,EI]
16. Liu, Y., Wu, H. C., Chhuan, M., Terrell, J. L., Tsao, C. Y., Bentley, W. E., Payne, G. F. (2015). Functionalizing soft matter for molecular communication. ACS Biomaterials Science and Engineering, 1(5), 320–328.
17. Luo, X. L., Tsao C. Y., Wu, H. C., Quan, D. N, Payne, G. F, Rubloff, G. W, Bentley, W. E. (2015). Distal modulation of bacterial cell-cell signalling in a synthetic ecosystem using partitioned microfluidics. Lab on a Chip, 15(8), 1842-1851. [SCI] 
18. Luo, X. L., Wu, H. C., Betz, J., Rubloff, G. W., Bentley, W. E. (2014). Air bubble-initiated biofabrication of freestanding, semi-permeable biopolymer membranes in PDMS microfluidics. Biochemical Engineering Journal, 85, 2-9.  [SCI, EI]
19. Wu, H. C., Tsao, C. Y., Quan, D. N., Cheng, Y., Servinskyd, M. D., Carter, K. K., Jee, J. J., Terrell, J. L., Zargar, A., Rubloff, G. W., Payne, G. F., Valdese, J. J., Bentley, W. E. (2013). Autonomous bacterial localization and gene expression based on nearby cell receptor density. Molecular Systems Biology, 22, 9:636. Featured article. [SCI]
20. Wu, H. C., Hebert, C. G., Hung, C. W., Quan, D. N., Carter, K. K., Bentley, W. E. (2013). Tuning cell cycle of insect cells for enhanced protein production. Journal of Biotechnology, 168(1):55-61. [SCI; EI]
21. Fernandes, R., Roy, V., Wu, H. C., Bentley, W. E. (2010). Engineered biological nanofactories trigger quorum sensing response in targeted bacteria. Nature Nanotechnology, 5(3), 213-217. [ SCI]
22. Luo, X. L., Wu, H. C., Tsao, C. Y., Cheng, Y., Betz, J., Payne, G. F., Rubloff, G. W., Bentley, W. E. (2012). Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling. Biomaterials, 33(20):5136-43. [SCI, EI]
23. Adams, B., Carter, K. K., Guo, M., Wu, H. C., Tsao, C. Y., Sintim, H., Valdes, J. J., Bentley, W. E. (2014). Evolved quorum sensing regulator, LsrR, for altered switching functions. ACS Synthetic Biology, 3 (4), 210–219  [SCI]
24. Betz, J.F., Cheng, Y., Tsao, C.Y., Zargar, A., Wu, H. C., Luo, X., Payne, G. F., Bentley, W. E., Rubloff, G. W. (2013). Optically clear alginate hydrogels for spatially controlled cell entrapment and culture at microfluidic electrode surfaces. Lab on a Chip, 13(10), 1854-1858. [ SCI]
25. Payne, G. F, Kim, E., Cheng, Y., Wu, H. C., Ghodssi, R., Rubloff, G. W., Raghavan, S. R., Culver, J. N., Bentley, W. E. (2013) Accessing biology's toolbox for the mesoscale biofabrication of soft matter. Soft Matter, 9, 6019-6032. [SCI, EI]
26. Cheng, Y., Tsao, C. Y., Wu, H. C., Luo, X. L., Terrell, J. L., Betz, J., Payne, G. F., Bentley, W. E., Rubloff, G. W. (2012). Electroaddressing functionalized polysaccharides as model biofilms for interrogating cell signaling. Advanced Functional Materials, 22(3), 519-528. [SCI, EI]
27. Liu, Y., Terrell, J. L., Tsao, C. Y., Wu, H. C., Javvaji, V., Kim, E., Cheng, Y., Wang, Y., Ulijn, R. V., Raghavan, S. R., Rubloff, G. W., Bentley,W. E., Payne, G. F. (2012). Biofabricating multifunctional soft matter with enzymes and stimuli-responsive materials. Advanced Functional Materials, 22(14), 3004-3012. [SCI, EI]
28. Kim, Y. W., Sardari, S. E., Meyer, M. T., Iliadis, A. A., Wu, H. C., Bentley, W. E, Ghodssi, R. (2012). An ALD aluminum oxide passivated surface acoustic wave sensor for early biofilm detection. Sensors and Actuators B-Chemical, 163(1), 135-145. [SCI, EI]
29. Terrell, J. L., Gordonov T., Cheng Y., Wu H. C., Sampey D., Luo X., Tsao C. Y., Ghodssi R., Rubloff G. W., Payne G. F., Bentley W. E. (2012). Integrated biofabrication for electro-addressed in-film bioprocessing. Biotechnology Journal, 7(3), 428-439. [SCI, EI]
30. Cheng, Y., Luo, X., Tsao, C. Y., Wu, H. C., Betz, J., Payne, G. F., Bentley, W. E., Rubloff, G. W. (2011). Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation. Lab on a Chip, 11(14), 2316-2318. [SCI]
31. Liu, Y., Cheng, Y., Wu, H. C., Kim, E., Ulijn, R. V., Rubloff, G. W., Payne, G. F. (2011). Electroaddressing agarose using Fmoc-phenylalanine as a temporary scaffold. Langmuir, 27(12), 7380-7384. [SCI, EI]
32. Tsao, C. Y., Hooshangi, S., Wu, H. C., Valdes, J. J., Bentley, W. E. (2010). Autonomous induction of recombinant proteins by minimally rewiring native quorum sensing regulon of E. coli. Metabolic Engineering, 12(3), 291-297. [SCI, EI]
33. Wu, H. C., Shi, X. W., Tsao, C. Y., Lewandowski, A. T., Fernandes, R., Hung, C. W., DeShong, P., Kobatake, E., Valdes, J. J., Bentley, W. E. (2009). Biofabrication of antibodies and antigens via IgG-binding domain engineered with activatable pentatyrosine pro-tag. Biotechnology and Bioengineering, 103(2), 231-240. [SCI, EI]
34. Shi, X. W., Liu, Y., Lewandowski, A. T., Wu, L. Q., Wu, H. C., Ghodssi, R., Payne, G. F. (2008). Chitosan biotinylation and electrodeposition for selective protein assembly. Macromolecular Bioscience, 8(5), 451-457. [SCI, EI]
35. Shi, X. W., Wu, H. C., Liu, Y., Tsao, C. Y., Wang, K., Kobatake, E., Payne, G. F. (2008). Chitosan fibers: versatile platform for nickel-mediated protein assembly. Biomacromolecules, 9(5), 1417-1423. [SCI, EI]
36. Hung, Y. J., Smolyaninov, II, Wu, H. C., Davis, C. C. (2006). Fluorescence enhancement by surface gratings. Optics Express, 14(22), 10825-10830. [SCI, EI]
37. Tso, I. M., Wu, H. C., Hwang, I. R. (2005). Giant wood spider Nephila pilipes alters silk protein in response to prey variation. Journal of Experimental Biology, 208(6), 1053-1061. [SCI]
38. Sheu, H. S., Phyu, K. W., Jean, Y. C., Chiang, Y. P., Tso, I. M., Wu, H. C., Ferng, S. L. (2004). Lattice deformation and thermal stability of crystals in spider silk. International Journal of Biological Macromolecules, 34(5), 325-331. [SCI]