NTUBST2019

• 2014 – 2020 德國慕尼黑工業大學 化學 博士

細胞內膜重塑和脂質平衡實驗室 (The Laboratory of Cellular Membrane Remodeling and Lipid Homeostasis) 致力於研究細胞內膜的動態平衡調節和脂質組成調控的複雜過程，尤其關注自噬 (autophagy)、脂質儲存與動員 (lipid storage and mobilization) 以及細胞質量控制 (cellular quality control) 這些重要機制之間的相互調控。

 

自噬是一種降解途徑，包括選擇性地清除和回收細胞內過剩或受損的成分，以維持細胞內衡定。這個過程涉及廣泛的膜重塑來形成自噬體 (autophagosomes)，一種將受質包裹與細胞質隔離的雙層膜囊泡。而後成熟的自噬體將與細胞內的降解胞器（如溶酶體或液泡）融合來達成最終的受質降解。我們研究自噬、膜重塑和脂質代謝之間的相互關係，以了解脂質分子和膜動態如何影響自噬的功能。通過對於這些機制的了解，我們將努力揭示自噬在維持細胞健康中所扮演的角色以及當自噬功能喪失所衍伸的相關的疾病（包括癌症和神經退行性疾病） 。 

 

實驗室的另一個重點研究領域是脂質儲存和動員。我們研究脂質儲存和釋放的分子機制，以脂滴 (lipid droplets) 做為重點研究對象。脂滴是特殊的細胞器，以中性形式儲存脂質，包括三酸甘油酯和固醇酯。我們的目標是了解脂滴如何作為脂質代謝、能量儲存和信號通路的動態樞紐。此外，我們亦將研究脂質如何在細胞內的運輸，特別是發生在細胞胞器間膜接觸點 (inter-organelle membrane contact sites) 的運輸，並探索脂質運輸異常在代謝性疾病發展中的角色。

 

細胞質量控制機制對於維護膜完整性、防止損壞或錯誤折疊的蛋白質在膜上的積聚至關重要。我們實驗室研究脂質膜和脂質-蛋白質相互作用在質量控制途徑中所扮演的角色，包括內質網相關的降解（ERAD）和未摺疊蛋白質反應（UPR）等質量控制機制。我們專注於解析脂質組成、膜動態和脂質代謝如何調控質量控制，特別是當細胞遭遇到脂毒性環境 (lipotoxic conditions) 或處於其他代謝背景下。 

 

我們利用酵母菌Saccharomyces cerevisiae和哺乳動物細胞系作為模型，結合先進的顯微鏡技術、脂質組學和基因操作等技術，來解決這些領域中的重要問題。我們提倡協作環境，使同學能夠與德國（Wilfling實驗室在MPIBP）和美國（Texas A&M大學的Sui實驗室）的研究團隊合作。這樣的合作將為學生提供早期國際研究學習的機會。我們熱忱歡迎有興趣出國留學的同學加入我們的實驗室，成為這些豐富機會的一份子！

 

• 國立臺灣大學 生化科技學系 助理教授 (2023/08～now)
• 2022 – 2023 Research Fellow, Cell Biology Program, Memorial Sloan Kettering Cancer Center, United States.
• 2021 – 2022 Postdoctoral Fellow, Department of Molecular Metabolism, Harvard T.H. Chan School of Public Health & Department of Cell Biology, Harvard Medical School, Harvard University, United States.

• 2020 Junior Scientists' Publication Award (Max Planck Institute of Biochemistry)

# Equal contribution

* Corresponding author

1. K Wang, CW Lee, X Sui, S Kim, S Wang, AB Higgs, AJ Baublis, GA Voth, M Liao*, TC Walther*, and RV Farese Jr.* (2023). The structure of phosphatidylinositol remodeling MBOAT7 reveals its catalytic mechanism and enables inhibitor identification. Nat. Commun., 14, 3533 (Citations ≥ 3, IF: 17.694, 6/204=2.9% in Biochemistry, Genetics and Molecular Biology (all) (Q1)) 
2. X Sui, K Wang, K Song, C Xu, J Song, CW Lee, M Liao, RV Farese Jr.*, and TC Walter* (2023). Mechanism of action for small molecule inhibitors of triacylglycerol synthesis. Nat. Commun., 14, 3100 (IF: 17.694, 6/204=2.9% in Biochemistry, Genetics and Molecular Biology (all) (Q1)) 
3. A Bieber^#, C Capitanio^#, PS Erdmann*, F Fiedler, F Beck, CW Lee, D Li, G Hummer, BA Schulman*, W Baumeister*, and F Wilfling* (2022). In situ structural analysis reveals membrane shape transitions during autophagosome formation. Proc. Natl. Acad. Sci. USA. 119 (39), e2209823119 (Citations ≥ 19, IF: 12.777, 4/110=3.6% in Multidisciplinary (Q1))
4. J Song, A Mizrak, CW Lee, M Cicconet, ZW Lai, WC Tang, CH Lu, SE Mohr, RV Farese Jr.*, and TC Walther* (2022). Identification of two pathways mediating protein targeting from ER to lipid droplets. Nat. Cell Biol. 24, 1364–1377. (Citations ≥ 13, IF: 28.213, 6/276=2.2% in Cell Biology (Q1))
5. S Qiao, CW Lee^#, D Sherpa^#, J Chrustowicz^#, J Cheng, M Duennebacke, B Steigenberger, O Karayel, DT Vu, SV Gronau, M Mann, F Wilfling, and BA  Schulman* (2022). Cryo-EM structures of Gid12-bound GID E3 reveal steric blockade as a mechanism inhibiting substrate ubiquitylation. Nat. Commun. 13 (1), 3041. (Citations ≥ 3, IF: 17.694, 6/204=2.9% in Biochemistry, Genetics and Molecular Biology (all) (Q1))
6. F Wilfling*^#, CW Lee^#, PS Erdmann*, and W Baumeister* (2021). Autophagy ENDing unproductive phase-separated endocytic protein deposits. Autophagy 17 (10), 3264-3265. (IF: 13.391, 21/279=7.5% in Cell Biology (Q1))
7. F Wilfling*^#, CW Lee^#, PS Erdmann*, Y Zheng, D Sherpa, S Jentsch, B Pfander, BA Schulman, and W Baumeister* (2020). A selective autophagy pathway for phase-separated endocytic protein deposits. Mol. Cell 80 (5), 764–778. (Citations ≥ 70, IF: 17.970, 10/274=3.6% in Cell Biology (Q1))
8. M Allegretti^#, CE Zimmerli^#, V Rantos, F Wilfling, P Ronchi, HKH Fung, CW Lee, W Hagen, B Turonova, K Karius, X Zhang, C Müller, Y Schwab, J Mahamid, B Pfander*, J Kosinski*, and M Beck* (2020). In-cell architecture of the nuclear pore and snapshots of its turnover. Nature 586, 796–800. (Citations ≥ 114, IF: 69.504, 1/110=0.9% in Multidisciplinary (Q1))
9. CW Lee^#, F Wilfling^#, P Ronchi, M Allegretti, S Mosalaganti, S Jentsch, M Beck*, and B Pfander* (2020). Selective autophagy degrades nuclear pore complexes. Nat. Cell Biol. 22 (2), 159-166. (Citations ≥ 69, IF: 28.213, 6/276=2.2% in Cell Biology (Q1))
10. S. Albert, W Wietrzynski^#, CW Lee^#, M Schaffer^#, F Beck, JM Schuller, PA Salomé, JM Plitzko, W Baumeister*, and BD Engel* (2019). Direct visualization of degradation microcompartments at the ER membrane. Proc. Natl. Acad. Sci. USA. 117 (2), 1069-1080. (Citations ≥ 63, IF: 12.777, 4/110=3.6% in Multidisciplinary (Q1))
11. CW Lee, FC Yang, HY Chang, H Chou, BCM Tan, and SC Lee* (2014). Interaction between salt-inducible kinase 2 and protein phosphatase 2A regulates the activity of calcium/calmodulin-dependent protein kinase I and protein phosphatase methylesterase-1. J. Biol. Chem. 289 (30), 21108-21119. (Citations ≥ 15, IF: 5.486, 68/415=16.4% in Biochemistry (Q1))
12. CM Wen*, CW Lee, CS Wang, YH Cheng, and HY Huang (2008). Development of two cell lines from Epinephelus coioides brain tissue for characterization of betanodavirus and megalocytivirus infectivity and propagation. Aquaculture 278 (1-4), 14-21. (Citations ≥ 110, IF: 5.135, 19/224=8.4% in Aquatic Science (Q1))