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Faculty

Professor Shih-Chung Chang
  • 發布單位:Department of Biochemical Science and Technology
Info


Professor Shih-Chung Chang

Shih-Chung Chang

Title Professor
Education Ph.D., Institute of Agricultural Chemistry, National Taiwan University
Research Expertise Cell Biology, Molecular Biology, Biochemistry
LAB Cell Biology Lab. (AC2-515)
TEL +886-2-3366-3740
E-mail shihchung@ntu.edu.tw
Personal webpage  

Research


  • Ubiquitin-proteasome degradation system
  • Post-translational modification systems of ubiquitin-like proteins
  • The molecular determinants and substrate specificities of ubiquitin-like protein proteases

Publications


  1. Hsiao CC, Chiang YW, Chao TL, Tsai ZU, Wang TX, Jiang YW, Hsu HF, Lu DC, Wang JT, Wang JR*, Wang AB*, Chang SY*, and Chang SC*. Simultaneous detection of antibody responses to multiple SARS-CoV-2 antigens by a Western blot serological assay. Appl Microbiol Biotechnol. 2022 Nov 21. doi: 10.1007/s00253-022-12288-0.(*Corresponding author) (SCI)
  2. Lai GC, Chao TC, Lin SY, Kao HC, Tsai YM, Lu DC, Chiang YW, Chang SY*, Chang SC*. Neutralization or enhancement of SARS-CoV-2 infection by a monoclonal antibody targeting a specific epitope in the spike receptor-binding domain. Antiviral Res. 2022; 200:105290. (*Corresponding author) (SCI)
  3. Li CJ, Chao TL, Chang TY, Hsiao CC, Lu DC, Chiang YW, Lai GC, Tsai YM, Fang JT, Ieong S, Wang JT, Chang SY*, Chang SC*. Neutralizing monoclonal antibodies inhibit SARS-CoV-2 infection through blocking membrane fusion. Microbiol Spectr. 2022; e0181421. (*Corresponding author) (SCI)
  4. Li CJ, Huang PH, Chen HW, Chang SC* (2021) Development and characterization of mouse monoclonal antibodies targeting to distinct epitopes of Zika virus envelope protein for specific detection of Zika virus. Appl Microbiol Biotechnol. 105(11):4663-4673. (*Corresponding author) (SCI)
  5. Chiang YW, Li CJ, Su HY, Hsieh KT, Weng CW, Chen HW, Chang SC*. Development of mouse monoclonal antibody for detecting hemagglutinin of avian influenza A(H7N9) virus and preventing virus infection. Appl Microbiol Biotechnol. 2021; 105(8):3235-3248. (*Corresponding author) (SCI)
  6. Cheng YC, Chang SC*. Development and biochemical characterization of the monoclonal antibodies for specific detection of the emerging H5N8 and H5Nx avian influenza virus hemagglutinins. Appl Microbiol Biotechnol. 2021; 105(1):235-245. (*Corresponding author) (SCI)
  7. Shin YC, Chen JH, Chang SC*. The molecular determinants for distinguishing between ubiquitin and NEDD8 by USP2. Scientific Reports. 2017; 7: 2304. (SCI)
  8. Lin YC, Chang SC*, Juang RH*. Plastidial alpha-glucan phosphorylase 1 complexes with disproportionating enzyme 1 in Ipomoea batatas storage roots for elevating malto-oligosaccharide metabolism. PLoS ONE. 2017; 12(5): e0177115.(SCI)
  9. Hu, CJ, Chien, CY, Liu, MT, Fang, ZS, Chang, SY, Juang, RH, Chang, SC, Chen, HW. Multi-antigen avian influenza a (H7N9) virus-like particles: particulate characterizations and immunogenicity evaluation in murine and avian models. BMC Biotechnol 2017; 17(1): 2.(SCI)
  10. Liu CY, Lu DC, Jiang YW, Yen YK, Chang SC*, and Wang AB* (2016). Easy and fast western blotting by thin-film direct coating with suction. Anal Chem. Jun 21;88(12):6349-56. (SCI).
  11. He JL, Chiu YC, Chang SC, Wang CH, Juang RH. Glycosylation at hemagglutinin Asn-167 protects the H6N1 avian influenza virus from tryptic cleavage at Arg-201 and maintains the viral infectivity. Virus Res 2015; 197: 101-7.(SCI)
  12. Young GH, Huang TM, Wu CH, Lai CF, Hou CC, Peng KY, Liang CJ, Lin SL, Chang SC, Tsai PR, Wu KD, Wu VC, Ko WJ. Hemojuvelin modulates iron stress during acute kidney injury: improved by furin inhibitor. Antioxid Redox Signal 2014; 20(8): 1181-94.(SCI)
  13. Yen YK, Jiang YW, Chang SC, Wang AB. Western blotting by thin-film direct coating. Anal Chem 2014; 86(10): 5164-70.(SCI)
  14. Huang TF, Cho CY, Cheng YT, Huang JW, Wu YZ, Yeh AY, Nishiwaki K, Chang SC, Wu YC. BLMP-1/Blimp-1 regulates the spatiotemporal cell migration pattern in C. elegansPLoS Genet 2014; 10(6): e1004428.(SCI)
  15. Sun CY, Chang SC, Wu MS. Uremic toxins induce kidney fibrosis by activating intrarenal renin-angiotensin-aldosterone system associated epithelial-to-mesenchymal transition. PLoS One. 2012; 7(3): e34026. (SCI)
  16. Sun CY, Chang SC, Wu MS. Suppression of Klotho expression by protein-bound uremic toxins is associated with increased DNA methyltransferase expression and DNA hypermethylation. Kidney Int.  2012; 81(7): 640-50. (SCI)
  17. Lin YC, Chen HM, Chou IM, Chen AN, Chen CP, Young GH, Lin CT, Cheng CH, Chang SC, Juang RH. Plastidial starch phosphorylase in sweet potato roots is proteolytically modified by protein-protein interaction with the 20S proteasome. PLoS One. 2012; 7(4): e35336. (SCI)
  18. Shin YC, Tang SJ, Chen JH, Liao PH, Chang SC. The molecular determinants of NEDD8 specific recognition by human SENP8. PLoS One. 2011; 6(11): e27742.(SCI)
  19. Nguyen TT, Chang SC, Evnouchidou I, York IA, Zikos C, Rock KL, Goldberg AL, Stratikos E, Stern LJ. Structural basis for antigenic peptide precursor processing by the endoplasmic reticulum aminopeptidase ERAP1. Nat Struct Mol Biol. 2011; 18(5): 604-13. (SCI)
  20. Liu LR, Lin SP, Chen CC, Chen YJ, Tai CC, Chang SC, Juang RH, Tseng YW, Liu BH, Mersmann HJ, Shen TL, Ding ST. Serum Amyloid A Induces Lipolysis by Downregulating Perilipin Through ERK1/2 and PKA Signaling Pathways. Obesity (Silver Spring). 2011. (SCI)
  21. Shin YC, Liu BY, Tsai JY, Wu JT, Chang LK, Chang SC. Biochemical characterization of the small ubiquitin-like modifiers of Chlamydomonas reinhardtii. Planta. 2010; 232(3): 649-62. (SCI)
  22. Rabl J, Smith DM, Yu Y, Chang SC, Goldberg AL, Cheng Y. Mechanism of gate opening in the 20S proteasome by the proteasomal ATPases. Mol Cell. 2008; 30(3): 360-8. (SCI)
  23. Evnouchidou I, Momburg F, Papakyriakou A, Chroni A, Leondiadis L, Chang SC, Goldberg AL, Stratikos E. The internal sequence of the peptide-substrate determines its N-terminus trimming by ERAP1. PLoS One. 2008; 3(11): e3658. (SCI)
  24. Smith DM, Chang SC, Park S, Finley D, Cheng Y, Goldberg AL. Docking of the proteasomal ATPases' carboxyl termini in the 20S proteasome's alpha ring opens the gate for substrate entry. Mol Cell. 2007; 27(5): 731-44. (SCI)
  25. Chang SC, Momburg F, Bhutani N, Goldberg AL. The ER aminopeptidase, ERAP1, trims precursors to lengths of MHC class I peptides by a "molecular ruler" mechanism. Proc Natl Acad Sci U S A. 2005; 102(47): 17107-12. (SCI)
  26. Draenert R, Le Gall S, Pfafferott KJ, Leslie AJ, Chetty P, Brander C, Holmes EC, Chang SC, Feeney ME, Addo MM, Ruiz L, Ramduth D, Jeena P, Altfeld M, Thomas S, Tang Y, Verrill CL, Dixon C, Prado JG, Kiepiela P, Martinez-Picado J, Walker BD, Goulder PJ. Immune selection for altered antigen processing leads to cytotoxic T lymphocyte escape in chronic HIV-1 infection. J Exp Med. 2004; 199(7): 905-15. (SCI)
  27. York IA, Chang SC, Saric T, Keys JA, Favreau JM, Goldberg AL, Rock KL. The ER aminopeptidase ERAP1 enhances or limits antigen presentation by trimming epitopes to 8-9 residues. Nat Immunol. 2002; 3(12): 1177-84. (SCI)
  28. Saric T, Chang SC, Hattori A, York IA, Markant S, Rock KL, Tsujimoto M, Goldberg AL. An IFN-gamma-induced aminopeptidase in the ER, ERAP1, trims precursors to MHC class I-presented peptides. Nat Immunol. 2002; 3(12): 1169-76. (SCI)
  29. Chen HM, Chang SC, Wu CC, Cuo TS, Wu JS, Juang RH. Regulation of the catalytic behaviour of L-form starch phosphorylase from sweet potato roots by proteolysis.Physiol Plant. 2002; 114(4): 506-15. (SCI)
  30. Chang SC, Lin PC, Chen HM, Wu JS, Juang RH. The isolation and characterization of Chaperonin 60 from sweet potato roots - Involvement of the chaperonins in starch biosynthesis. Bot Bul Acad Sin. 2000; 41(2): 105-11. (SCI)

Courses Taught


  • Enzyme Biochemistry Laboratory
  • Biochemistry
  • Biochemistry Laboratory
  • Biotech Core Techniques(BCT)
  • Research Methods in Bioscience and Biotechnology
  • Introduction to Biotechnology
  • Biochemistry Exploration/ Advanced Biochemistry Exploration