Orbit

Ja-An Annie Ho
Full-Time,Professor,Bioindustry
Job Title Professor
Name Ja-An Annie Ho
Office Tel No. 02-3366-4438
Email jaho@ntu.edu.tw
Education Doctor, Cornell University(1994~1998)
Research Expertise Biosensor development, Targeted drug delivery system development, Bioanalytical chemistry
Location BioAnalytical Chemistry and Nanobiomedicine Lab.(AC2-307)
Tel(Lab) +886-2-3366-4439
Lab Website https://sites.google.com/site/hotliposome/
  • Doctor, Cornell University(1994/08 ~ 1998/08)
  • Master. University of Wisconsin(1992/08 ~ 1994/08)

歡迎對研發『生醫感應器』、『靶向藥物傳遞系統』及『生化分析』研究感興趣的同學加入我們的行列!!

Prof. Ja‐an Annie Ho and Hot liposome group welcome to all, those who are interested in biosensor development, targeted drug delivery system development as well as in bioanalytical chemistry, to join us.

研究興趣

本人與研究團隊成員的研究興趣涵蓋三大重點,包括:分析化學生物科技奈米科技

分析化學方面研究

本人的研究興趣為研發快速偵檢的感測系統,可應用的範圍從臨床醫事的生化值檢驗,反恐生化毒劑的快速偵檢,以及食品和環境安全的監測。新開發出的微脂球型免疫分析系統,或是奈米材料輔助之體外免疫檢驗試劑組(in vitro diagnostic kit)應能成功地取代耗費多日、感度有限的傳統鑑定方式,加速並加強監測,以落實保障國人健康與安全。

利用包裹具特殊光、電活性標記分子的微脂體(liposome 如圖一)為訊息放大劑(signal amplifier)可應用在微脂體免疫檢驗法 (LIA: liposome immunoassay)。這些檢定法的理論基礎乃建立在被檢測物與其抗體間特異專有的免疫性辨識結合作用(immunorecognition reaction),並利用由微脂體中釋出的標記分子達到放大訊號的目的。不同於酵素免疫檢驗法(ELISA: enzyme‐linked immunosorbent assay)需要多次更換試劑並耗時等待酵素與其基質作用),微脂體之破裂所釋放出的標記分子提供了『立即式』的訊號放大(instaneous signal amplification)。

 

 
圖一 Preparation and structure of liposomes

 

 

本人及研究團隊針對具有不同光、電活性的標記分子進行研究─從最普通的染劑分子(such as, sulforhodamine, carboxyfluorescein or methyl blue),到可發冷光的水母蛋白分子(such as, aequorin)及電化學活性分子(such as, potassium ferrocyanide),發展不同型式的免疫生化感測器。
Highlights I–III provide a summary of Dr. Ho’s research in the field of Analytical Chemistry.

Highlight I Liposomes in Flow Injection Systems

  1. Application of Liposomal Bioluminescent Label in the Development of Flow Injection ImmunoAnalytical System [Anal. Chem200577 (11), 3431–3436]; illustrated in Figure 2.
  2. Application of Ganglioside‐Sensitized Liposomes in a Flow Injection Immunoanalytical System for the Determination of Cholera Toxin [Anal. Chem.200779 (1), 246–250].

 

 

圖二 (A) Schematic representation of a Flow‐Injection Liposome Immunoanalytical (FILIA) system with bioluminescence detection. (B) Reproducibility of the bioluminescence signal.

 

Highlight II Development of Electrochemial Immunosensors

  1. Electrochemical Immunosensor for Cholera Toxin Using Liposomes and poly(3,4‐ ethylenedioxythiophene)‐Coated Carbon Nanotubes [Anal. Chem.200678 (4), 1115–1121]; illustrated in Figure 3.
  2. Dual Electrochemical Determination of Glucose and Insulin Using Enzyme and Ferrocene Microcapsules [Biosens. Bioelectron200722, 1147–1153]; illustrated in Figure 4.

 

 
 

 

圖三 (A) Schematic representation of an electrochemical immunosensor for cholera toxin. (B) Calibration plot for cholera toxin. Inset: Linear portion of the main curve.

 

圖四 Schematic representation of the encapsulation, biolabeling, and detection of glucose and insulin.

 

Highlight III Liposomes in Immunoaffinity Chromatographic Biosensors

  1. Using Liposomal Fluorescent Biolabels To Develop an Immunoaffinity Chromatographic Biosensing System for Biotin [Anal. Chem200880 (16), 6405–6409]; illustrated in Figure 5.

圖五 Schematic representation of the operation of an immunosensor for the detection of biotin.

 

生物科技方面研究

本人選擇以微脂體(liposome)為其研究重心,除了分析檢驗上的應用,另一研究方向為極具挑戰性的奈米型藥物傳遞系統(nano Drug Delivery System, nDDS)的研發。由於 DDS 具備大幅增進現有藥物療效與價值的潛力,因而深受學界、業界長期以來的重視。相較於傳統醫療的經血液循環、致使藥物分散於非病灶之身體各器官的顧慮,DDS 可以被設計為具特定靶向功能之載體,並被相信可增強特定藥物對於標的器官的療效,大幅減低副作用。微脂球(liposome)是 DDS 概念的先驅者,為少數被美國 Food and Drug Administration 核可使用於臨床的藥物載體之一,其生化相容性及生物可分解性已被確認。微脂球可被應用於化療藥物的載體(如Lipo‐Dox),目前已被使用在臨床醫療上;透過靜脈滴注,可選擇性地殺滅癌細胞。本人的團隊在此類研究的貢獻即是利用表面修飾特定抗體的微脂體對於特定癌症標識分子的辨識能力,藉由表面修飾化學以提高微脂體的載藥量,並得以調控藥物釋放之條件與速率,將藥物傳送到病灶,再經由細胞膜融合或胞飲作用將藥物導入病灶細胞之內,達到兼具靶向、高載藥量、以及可調節釋放速率的藥物輸送之目的。此外,本人更藉由與免疫治療 (immuno‐ therapy)專家暨南大學應化系/生醫所 吳立真教授的合作,成功地改善liposome在基因轉殖上的效率。這個成果對於免疫/基因治療的診療效益有十分正面的影響。
此外值得一提的是,吳教授與本人在肝癌/肝硬化治療的合作研究成果使他們贏得 UC‐Davis Professor M. Eric Gershwin (MD, PhD) 的邀請,與其他國際知名團隊聯合出版一本參考書籍(Spirulina in Human Nutrition and Health),該書已於本年度出版(出版商CRC press, Botan Roca, LA ) (如圖六)。微脂體技術在生醫奈米領域研究所累積的合作成果已於日前投稿於癌症治療相關的國際知名期刊。

 

 

圖六 Cover of the book Spirulina in Human Nutrition and Health (Publication Date: 2007‐10‐08. ISBN/ASIN: 142005256X)
 

奈米科技方面研究
走在國際研究趨勢的前端,本人與研究團隊全體藉由各種奈米粒子的合成與修飾,改善增強其在分析化學或藥物傳遞與治療的效率。圖九所示,即為利用奈米金所修飾的網版印刷電極 (screen printed electrode) 以期達到電化學分析表現的改善 (請見圖七)。

 

Highlight IV Nanomaterial‐Modified Electrochemical Sensors

  1. An Attomole DNA Electrochemical Sensor for the Detection of Escherichia coli O157 (Anal. Chem200981 (7), 2470–2476).
  2. Carbon Nanoparticle‐Enhanced Immunoelectrochemical Detection for Protein Tumor Marker With CdS Biotracers (Anal. Chem200981(4), 1340‐1346); illustrated in Figures 8.

 

 

圖七 SEM images of the surfaces of (A) a gold nanoparticle (nanoAu)‐structured screen‐ printed electrode (SPE) after electrodeposition and (B) the bare SPE prior to electrodeposition.

 

 

圖八 Cartoon representation of the assembly and amplified detection of carcinoembryonic antigen (CEA) using the anti‐CEA‐CdS biotracer and the carbon nanoparticle/poly(ethylene imine) (PEI)‐modified screen‐printed graphite electrode (CNP–PEI/SPGE)

2015
  1. Chang, H.-C. and Ho, J.-a. A.* (2015) Gold Nanocluster-Assisted Fluorescent Detection for Hydrogen Peroxide and Cholesterol Based on the Inner Filter Effect of Gold Nanoparticles. Analytical Chemistry, 87(20), 10362-10367. (SCI) DOI: 10.1021/acs.analchem.5b02452
  2. Lee, M.-t. G, Wang, H.-M., Ho, J.-a. A., Fan, N.-C., Yang, Y.-L.,  Lee, C.-C.*, Chen, S.-C. (2015) Resuscitation Using Liposomal Vasopressin in an Animal Model of Uncontrolled Hemorrhagic Shock. Plos One, 10(7), e0130655. (SCI) DOI: 10.1371/journal.pone.0130655
  3. Chang, Y.-J., Hsu, S.-L., Liu, Y.-T., Lin, Y.-H., Lin, M.-H., Huang, S.-J., Ho, J.-a. A.; Wu, L.-C.* (2015) Gallic Acid Induces Necroptosis via TNF-alpha Signaling Pathway in Activated Hepatic Stellate Cells. Plos One, 10(3), e0120713. (SCI) DOI: 10.1371/journal.pone.0120713
  4. Lu, H.-Y., Chang, Y.-J., Fan, N.-C., Wang, L.-S., Lai, N.-C., Yang, C.-M., Wu, L.-C., Ho, J.-a. A.* (2015) Synergism through combination of chemotherapy and oxidative stress-induced autophagy in A549 lung cancer cells using redox-responsive nanohybrids: A new strategy for cancer therapy. Biomaterials, 42, 30-41. (SCI) DOI: 10.1016/j.biomaterials.2014.11.029
  5. Jou, A. F.-j., Lu, C.-H., Ou, Y.-C., Wang, S.-S., Hsu, S.-L., Willner I* and Ho, J.-a. A.* (2015) Diagnosing the miR-141 prostate cancer biomarker using nucleic acid-functionalized CdSe/ZnS QDs and telomerase. Chemical Science, 6, 659-665. (SCI) DOI: 10.1039/C4SC02104E
2014
  1. Chen, S.-H., Hsu, Y.-Pb, Lu, H.-Y., Ho, J.A.A.* (2014) Gold/Phospholipid nanoconstructs as label-free optical probes for evaluating phospholipase A2 activity. Biosensors and Bioelectronics, 52, 202-208. DOI: 10.1016/j.bios.2013.08.017
  2. Liao, W.-C., Ho, J.-a. A.* (2014) Improved activity of immobilized antibody by paratope orientation controller: Probing paratope orientation by electrochemical strategy and surface plasmon resonance spectroscopy. Biosensors and Bioelectronics, 55, 32-38. DOI: 10.1016/j.bios.2013.10.054
2013
  1. Chuang, M.-C., Lai, H.-Y., Ho, J.-a. A.*, Chen, Y.-Y. (2013) Multifunctional microelectrode array (mMEA) chip for neural-electrical and neural-chemical interfaces: Characterization of comb interdigitated electrode towards dopamine detection.Biosensors and Bioelectronics, 41, 602–607. DOI: 10.1016/j.bios.2012.09.030
  2. Tien, C.-Y., Jou, A.F.-J., Fan, N.-C., Chuang, M.-C., Ho, J.-A.A.* (2013) Preparation of Liposomal Progesterone and Its Application on the Measurement of Progesterone Interpreted via Electrochemical and Colorimetric Sensing Platforms.Electroanalysis, 25(4), 1017–1022. DOI: 10.1002/elan.201200563
  3. Teng, I.-T., Chang, Y.-J., Wang, L.-S., Lu, H.-Y., Wu, L.-C., Yang, C.-M., Chiu, C.-C., Yang, C.-H., Hsu, S.-L., Ho, J.-a. A.* (2013) Phospholipid-functionalized mesoporous silica nanocarriers forselective photodynamic therapy of cancer. Biomaterials, 34, 7462-7470. DOI: 10.1016/j.biomaterials.2013.06.001
  4. Tanwar, S.,* Ho, J.-a. A., Magi, E. (2013) Green synthesis and characterization of novel gold nanocomposites for electrochemical sensing applications. Talanta, 117, 352-358. DOI:10.1016/j.talanta.2013.09.011
  5. Liao, W.-C., Chuang, M.-C., Ho, J.-a. A.* (2013) Electrochemical sensor for multiplex screening of genetically modified DNA: Identification of biotech crops by logic-based biomolecular analysis. Biosensors and Bioelectronics, 50, 414-420. DOI: 10.1016/j.bios.2013.06.044
2012
  1. Ho, J.-a. A.*, Kuo, T.Y., Yu, W.G. (2012) Dual polarization interferometric and capillary electrophoretic analysis of supported lipid bilayer constructed on silica-based surface: Evaluation of its anti-protein adsorption effect. Analytica Chimica Acta, 714: 127-33.(SCI) DOI: 10.1016/j.aca.2011.11.062
  2. Ho, J.-a. A.*, Jou, A. F.J., Wu, L.C., Hsu, S.L. (2012) Development of an immunopredictor for the evaluation of the risk of cardiovascular diseases based on the level of soluble P-selectin. Methods, 56(2): 223-9.(SCI) DOI: 10.1016/j.ymeth.2011.10.014
  3. Prasad, K. S., Chuang, M.C.,  Ho J.-a. A.* (2012) Synthesis, characterization, and electrochemical applications of carbon nanoparticles derived from castor oil soot. Talanta, 88: 445-9.(SCI) DOI:10.1016/j.talanta.2011.10.056
2011
  1. Su, L.C., Chang, Y.F., Chou, C., Ho, J.-a. A., Li, Y.C., Chou, L.D., Lee, C.C. (2011). Binding kinetics of biomolecule interaction at ultralow concentrations based on gold nanoparticle enhancement. Analytical Chemistry, 83(9): 3290-6. (SCI) DOI:10.1021/ac1028616
  2. Jeng, C.C., Cheng, S.H., Ho, J.-a. A., Huang, S. H.-Y., Chang, J. C., Tsai, P.J., Yang, C.S., Lo, L.W. (2011) Dynamic probing of nanoparticle stability in vivo: a liposomal model assessed using in situ microdialysis and optical imaging. Journal of Nanomaterials, v.2011, 932719, DOI:10.1155/2011/932719
2010
  1. Ho, J.-a. A.*, Wu, L.C., Chang, L.H., Hwang, K.C, Hwa, J.-R. R. (2010). Liposome-based immunoaffinity chromatographic assay for the quantitation of immunoglobulin E in human serum. Journal of Chromatography B, 878(2), 172-176. (SCI) DOI: 10.1016/j.jchromb.2009.07.037
  2. Chou, C., Ho, J.-a. A., Chen, C.-C., Ming-Yaw, Liu, W.C., Chang, Y.F., Fu, C., Chen, S.H., Kuo, T.Y. Metal-Enhanced Fluorescence. Geddes, Chris D. (Ed), (2010) Chap 8, John Wiley & Sons press. ISBN: 978-0-470-22838-8  (Book Chapter)
  3. Ho, J.-a. A.*, Chang, H.C., Shih, N.Y.,Wu, L.C., Chang, Y.F., Chen, C.C., Chou, C. (2010) Diagnostic Detection of Human Lung Cancer-Associated Antigen Using a Gold Nanoparticle-Based Electrochemical Immunosensor. Analytical Chemistry, 82 (14), 5944–5950. (SCI) DOI: 10.1021/ac1001959
  4. Wang, L.S., Wu, L.C.*, Lu, S.Y., Chang, L.L., Teng, I.T, Yang, C.M.*, Ho, J.-a. A.* (2010) Biofunctionalized Phospholipid-Capped Mesoporous Silica Nanoshuttles for Targeted Drug Delivery: Improved Water Suspensibility and Decreased Nonspecific Protein Binding. ACS Nano, 4(8), 4371-4379. (SCI) DOI: 10.1021/nn901376h
  5. Chang, I.P, Hwang, K.C., Ho, J.-a. A., Lin, C.C., Hwa, J.-R. R., Horng, J.C. (2010) Facile surface functionalization of nanodiamonds. Langmuir, 26(5), 3685-3689. (SCI) DOI: 10.1021/la903162v
  6. Ho, J.-a. A.*, Hsua, W.L., Liao, W.C., Chiua, J.K., Chen, M.L., Chang, H.C., Lin, C.C. (2010) Ultrasensitive electrochemical detection of biotin using electrically addressable site-oriented antibody immobilization approach via aminophenyl boronic acid. Biosensors and Bioelectronics, 26(3), 1021-1027 (SCI) DOI: 10.1016/j.bios.2010.08.048
  7. Lu, C.L., Prasad K.S., Wu, H.L., Ho, J.-a. Annie, Huang, Michael H.*, (2010) Au Nanocube-Directed Fabrication of Au−Pd Core−Shell Nanocrystals with Tetrahexahedral, Concave Octahedral, and Octahedral Structures and Their Electrocatalytic Activity.  Journal of American Chemical Society, 132 (41), 14546–14553 (SCI) DOI: 10.1021/ja105401p
  8. Sahabuddina, Sk,Chang T.C., Lin, C.C., Jan F.D., Hsiao, H.Y., Huang, K.T., Chen, J.H. , Horng, J.C.,  Ho, J.-a. A, Lin, C.C.* (2010) Synthesis of N-modified sTn analogs and evaluation of their immunogenicities by microarray-based immunoassay. Tetrahedron, 66(38), 7510-7519 (SCI) DOI:10.1016/j.tet.2010.07.050
  9. Wu L.C., Jou, A. F.J., Chen, S.H., Tien, C.Y., Cheng, C.F., Fan, N.C., Ho, J.-a. A.* (2010) Antioxidant, anti-inflammatory and anti-browning activities of hot water extracts of oriental herbal teas. Food & Function, 1, 200-208 DOI: 10.1039/C0FO00047G
 
2009
  1. Ho, J.-a. A.*; Lin, Y.C.; Wang, L.S.; Hwang, K.C. and Chou, P.T. (2009). Carbon nanoparticle‐enhanced immunoelectrochemical detection for protein tumor marker with CdS biotracers. Analytical Chemistry, 81(4), 1340-1346. (SCI)DOI: 10.1021/ac801832h
  2. Liao, W. C., Ho, J.-a. A.* (2009). Attomole DNA electrochemical sensor for the detection of Escherichia coli O157Analytical Chemistry, 81(7), 2470-2476. (SCI) DOI: 10.1021/ac8020517
  3. Ho, J.-a A.*, Chiu, J.K., Hsu, W.L., Hong, J.C., Lin, C.C., Hwang, K.C. and Hwu, J.R. (2009). Gold-Nanostructured Immunosensor for the Electrochemical Sensing of Biotin Based on Liposomal Competitive Assay. Journal of Nanoscience and Nanotechnology, 9(4), 2324-2329. (SCI) DOI: 10.1166/jnn.2009.SE40
  4. Viswanathan, S., Rani, C., Vijay Anand, A., Ho, J.-a. A. (2009). Disposable electrochemical immunosensor for carcinoembryonic antigen using ferrocene liposomes and MWCNT screen-printed electrode. Biosensors and Bioelectronics, 24(7), 1984-1989. (SCI) DOI: 10.1016/j.bios.2008.10.006
  5. Wu, L.C.*; Chang, L.H.; Chen, S.H.; Fan, N.C.; Ho, J.-a. A.** (2009). Antioxidant activity and melanogenesis inhibitory effect of the acetonic extract of Osmanthus fragrans: A potential natural and functional food flavor additive. LWT - Food Science and Technology, 42(9), 1513-1519. (SCI) DOI:10.1016/j.lwt.2009.04.004
  6. Chien, H.T., Lee, C.K., Chiu, H.K., Hsu, K.C., Chen, C.C.,* Ho, J.‐a. A., Chou, C. (2009). The Comparison between the Graded Photonic Crystal Coupler and Various Coupler. Journal of Lightwave Technology, 27(14), 2570-2574. (SCI) DOI:10.1109/JLT.2008.2012271
  7. Ho, J.-a. A.*, Hung, C.H.,Wu, L.C., Liao, M.Y. (2009). Folic acid-anchored PEGgylated phospholipid bioconjugate and its application in a liposomal immunodiagnostic assay for folic acid. Analytical Chemistry, 81(14), 5671-5677. (SCI) DOI:10.1021/ac900402v
  8. Lin, P.C., Adak, A.K., Ueng. S.H., Huang, L.D., Huang, K.T., Ho, J.-a.A., Lin C.C. (2009) DAST-mediated regioselective anomeric group migration in sacchardies. Journal of Organic Chemistry, 74(11), 4041-4048. (SCI) DOI: 10.1021/jo900516r
  9. Hwu, J.R.*, Huang,  J.-T. J., Tsai, F.Y., Tsay, S.C., Hsu, M.H., Hwang, K.C., Horng, J.C., Ho, J.-a. A, Lin, C.C. (2009). Photochemical Activities of N-Nitroso Carboxamides and Sulfoximides and Their Application to DNA Cleavage, Chemistry - A European Journal, 15(35), 8742 - 8750 (SCI) DOI: 10.1002/chem.200802571
2008
  1. Ho, J-a A.*, Zeng, S.C., Tseng, W.H., Lin, Y.J. and Chen, C.H. (2008). Liposome-Based Immunostrip for the Rapid Detection ofSalmonella. Analytical and Bioanalytical Chemistry391(2), 479-485. (SCI) DOI: 10.1007/s00216-008-1875-5
  2. Hsin,Y.L., Lin, C.F., Liang, Y.C., Hwang, K.C., Horng, J.C., Ho, J-a A., Lin, C.C. and Hwu, J.R.(2008). Microwave Arcing Induced Formation and Growth Mechanisms of Core/Shell Metal/Carbon Nanoparticles in Organic Solutions. Advanced Functional Materials, 18(14), 2048-2056. (SCI) DOI: 10.1002/adfm.200701407
  3. Ho, J.-a A.* and Hung, C.H. (2008). Using Liposomal Fluorescent Biolabels To Develop an Immunoaffinity Chromatographic Biosensing System for Biotin. Analytical Chemistry, 80(16), 6405-6409. (SCI) DOI: 10.1021/ac800850w
2007
  1. Wu, L.C. and Ho, J-a A. In Spirulina in Human Nutrition and Health, Gershwin, M.E. and Belay, A. (Eds) (2007). Chap 6 (p119-152), CRC Press, Boca Raton, FL, USA. (Book Chapter)
  2. Subramanian, V., Liao, W.C., Huang, C.C., Hsu, W.L. and Ho, J-a A.* (2007). Rapid Analysis of L-dopa in Urine Samples Using Gold Nanoelectrode Ensembles. Talanta74, 229-234. (SCI) DOI: 10.1016/j.talanta.2007.05.056
  3. Kim, B.Y., Swearingen, C.B., Ho, J-a A., Romanova, E.V., Bohn, P.W. and Sweedler, J.V. (2007). Direct Immobilization of Fab’ in Nanocapillaries for Manipulating Mass-Limited Samples. Journal of American Chemical Society, 129(24), 7620-7626. (SCI) DOI: 10.1021/ja070041w
  4. Ho, J-a A.*, Wu, L.C., Huang, M.R., Lin, Y.J., Baeumner, A.J. and Durst, R.A. (2007). Application of Ganglioside-sensitized Liposomes in a Flow-Injection Immunoanalytical System for the Determination of Cholera Toxin. Analytical Chemistry, 79(1), 246-250. (SCI) DOI: 10.1021/ac060889n
  5. Subramanian, V. and Ho, J-a A.* (2007). Dual Electrochemical Determination of Glucose and Insulin Using Enzyme and Ferrocene Microcapsules. Biosensors and Bioelectronics, 22(6), 1147-1153. (SCI) DOI: 10.1016/j.bios.2006.07.031
  6. Ho, J-a A.*, Wu, L.C, Fan, N.C., Lee, M.S., Kuo, H.Y. and Yang, C.S. (2007). Development of a Long-life Capillary Enzyme Bioreactor for the Determination of Blood Glucose. Talanta, 71, 391-396. (SCI) DOI: 10.1016/j.talanta.2006.04.023
2006
  1. Subramanian, V., Wu, L.C., Huang, M.R. and Ho, J-a A.* (2006). Electrochemical Immunosensor for Cholera Toxin Using Liposomes and poly(3,4-ethylenedioxythiophene)- Coated Carbon Nanotubes. Analytical Chemistry, 78(4), 1115-1121. (SCI)DOI: 10.1021/ac051435d
  2. Ho, J-a A.*, Zeng, S.C., Huang, M.R. and Kuo, H.Y. (2006). Development of Liposomal Immunosensor for the Measurement of Insulin with Femtomole Detection. Analytica Chimica Acta, 556, 127-132. (SCI) DOI: 10.1016/j.aca.2005.08.074
  3. Wu, L.C.*, Hsu, H.W., Chen, Y.C., Chiu, C.C., Chang, C.W., Lin, Y.Y. and Ho, J-a A.* (2006). Antioxidant and Antiproliferative Activities of Red Pitaya. Food Chemistry, 95, 319-327. (SCI) DOI: 10.1016/j.foodchem.2005.01.002
  4. Ho, J-a A.*, Kuo, H.Y., Wang, Y.T. and Yang, C.S. (2006). Occurrence of Aristolochic Acids in Over-the-Counter Chinese Prepared Medicines. Asian Journal of Chemistry, 18(1)253-258. (SCI)
2005
  1. Ho, J-a A.* and Huang, M.R. (2005). Application of Liposomal Bioluminescent Label in the Development of Flow Injection Immunoanalytical System. Analytical Chemistry, 77(11), 3431-3436. (SCI) DOI: 10.1021/ac0484474
  2. Wu, L.C., Ho, J-a A., Shieh, M.C. and Lu, I.W. (2005). Antioxidant and Antiproliferative Activities of Spirulina and ChlorellaWater Extracts. Journal of Agricultural and Food Chemistry, 53(10), 4207-4212. (SCI) DOI: 10.1021/jf0479517
2004
  1. Lo, L.W., Ho, J-a A, Chang, Y.H., Chang, C.H, Yang, C.S. (2004). Potential Usage of Liposome-encapulated Phosphor for in vivo Imaging of Tissue Oxygenation. Biomedical Engineering Applications, Basis and Communications. 16(4), 224-229. (EI)
  2. Ho, J-a A.,* Hsu, H.W. and Huang, M.R. (2004). Liposome-based Microcapillary Immunosensor for Detection of Escherichia coli O157:H7. Analytical Biochemistry. 330:342-349. (SCI) DOI: 10.1016/j.ab.2004.03.038
2003
  1. Ho, J-a A.* and Hsu, H.W. (2003). Procedures for Preparing Escherichia coli O157:H7 Immunoliposome and Its Application in Liposome Immunoassay. Analytical Chemistry. 75:4330-4334. (SCI) DOI: 10.1021/ac0343580
  2. Wu, L.C., Chen, Y.C., Ho, J-a A. and Yang, C.S. (2003). Inhibitory Effect of Red Koji Extracts on Mushroom Tyrosinase.Journal of Agricultural and Food Chemistry. 51: 4240-4246. (SCI) DOI: 10.1021/jf034064f
  3. Ho, J-a A.* and Durst, R.A. (2003). Detection of Fumonisin B1: Comparison of Flow-injection Liposome Immunoanalysis with High-performance Liquid Chromatography. Analytical Biochemistry. 312:7-13. (SCI) DOI: 10.1016/S0003-2697(02)00393-7
2000-2002
  1. Ho, J-a A.* and Wauchope, R.D. (2002). A rapid strip liposome immunoassay for aflatoxin B1. Analytical Chemistry. 74:1493-1496. (SCI) DOI: 10.1021/ac010903q
  2. Ho, J-a A., Yang, C.S. and Tzeng, H.F. (2001). Overview of capillary electrochromatography. 化學. 59(3):399-408.
  3. Ho, J-a A.* and Durst, R.A. (2000). Preparation of reagents for the determination of fumonisin B1 by flow-injection immunoanalyis. Analytica Chimica Acta. 414:51-60. (SCI) DOI: 10.1016/S0003-2670(00)00782-0
  4. Ho, J-a A.* and Durst, R.A. (2000). Development of a flow-injection liposome immunoanalytical (FILIA) system for fumonisin B1.Analytica Chimica Acta. 414:61-69. (SCI) DOI: 10.1016/S0003-2670(00)00786-8