Associate Professor

Chii-Shen Yang  (EM Building)

Tel

(Office) 02-3366-2275

(Lab) 02-3366-3255

E-mail

chiishen@ntu.edu.tw

Web

CSY lab

Expertise

Structural biology

Physiology

Biophysics

Education

Doctor

University of Illinois at Chicago

Department of Physiology & Biophysics

1993 ~ 1998

Master

National Taiwan University

Department of Physiology

1989 ~ 1992

Bachelor

National Cheng Kung University

Department of Biology

1983 ~ 1987

 

Experience

Dept Biochem Sci Tech (BST)

Associate Professor

2013/08 ~ now

Dept Biochem Sci Tech (BST)

Assistant Professor

2005/08 ~ 2013/07

Center for Membrane Biology, U of Texas Houston Health Science Center

Post-doctoral Fellow

2003 ~ 2005

Dept Microbiology & Molecular Genetics, U of Texas-Houston

Post-doctoral Fellow

1999 ~ 2003

 

Current Teaching

Introduction to structural biology

Microbial biochemical technology

 Microbiology

 Structural biology and bioinformatics

 Theory and Practial protein crystallization

Seminar

Research study

 

Research Focus

 

A novel six-rhodopsin system in a single archaeon:
After four years of intensive study, our lab unveiled a six-rhodopsin photosensory system, the most in microbes, in Haloarcula maris mortui.  The system includes three ion translocating rhodopsins, two sensory rhodopsins and one sensory-like rhodopsin.

描述: 描述: 描述: 描述: Six-rhodopsin from H. marismortui
HmBRI: Light-driven proton outward translocator; Bacteriorhodopsin-like.
HmBRII: Light-driven proton outward translocator; Bacteriorhodopsin-like.
HmHR: Light-driven chloride inward translocator; Halorhodopsin-like.
HmSRI: Phototaxis sensory rhodopsin with a congnate transducer, HmHtrI.  Like those known as SRI.
HmSRII: Photo-repellent sensory rhodopsin with a cognate transducer, HmHtrII. Like those known as SRII.
HmSRM: Sensory-like rhodopsin working with a cognate transducer, HmHtrM. Instead of containing Histidine kinase binding domain found in any transducer associated with sensory rhodopsin, HmHtrM lacks such domain.

GPCR, RGS and G-protein signaling: basic scientific facts and biotechnological studies
Our lab is also working on G-protein related pathways in plant and higer systems. 
A GPCR drug screening system is under developments.

Biomimicry based on Photoreceptors and their associated proteins
Bacteriorhodopsins are light-driven proton translocators.  Biomimicry based on photoreceptors potentially can take advantage of sun light to drive the direct production of bioenergy or indirectly.  Bacteriorhodopsin, Halorhodopsin, Sensory rhodopsins, transducers and F1/Fo ATPase are the candidates.
描述: 描述: 描述: 描述: Beta-gamma subunits描述: 描述: 描述: 描述: RGS描述: 描述: 描述: 描述: G-alpha subunit

Publication  1998 ~ now

Original article

2013

1.           Fu HY, Yi HP, Lu YH, Yang CS. Insight into a single halobacterium using a dual-bacteriorhodopsin system with different functionally optimized pH ranges to cope with periplasmic pH changes associated with continuous light illumination. Mol Microbiol 2013; 88(3): 551-61.(SCI)

2.           Fu HY, Lu YH, Yi HP, Yang CS. A transducer for microbial sensory rhodopsin that adopts GTG as a start codon is identified in Haloarcula marismortui. J Photochem Photobiol B 2013; 121C: 15-22.(SCI)

3.           Hsu MF, Yu TF, Chou CC, Fu HY, Yang CS*, Wang AH*. Using Haloarcula marismortui Bacteriorhodopsin as a Fusion Tag for Enhancing and Visible Expression of Integral Membrane Proteins in Escherichia coli. PLoS ONE 2013; 8(2): e56363.(SCI) *: co-corresponding author

 

2012

1.           Jose Priya TA, Lin YH, Wang YC, Yang CS, Chang PS, Song YL. Codon changed immobilization antigen (iAg), a potent DNA vaccine in fish against Cryptocaryon irritans infection. Vaccine. 2012; 30(5): 893-903. (SCI)

2.           Huang PS, Yeh HS, Yi HP, Lin CJ, Yang CS. Fluorescence-based assay probing regulator of G protein signaling partner proteins. Anal Biochem. 2012; 423(1): 133-40. (SCI)

 

2011

1.         Zeng YF, Ko TP, Lai HL, Cheng YS, Wu TH, Ma Y, Chen CC, Yang CS, Cheng KJ, Huang CH, Guo RT, Liu JR. Crystal structures of Bacillus alkaline phytase in complex with divalent metal ions and inositol hexasulfate. J Mol Biol. 2011; 409(2): 214-24. (SCI)

 

2010

1.         Lin YC, Fu HY, Yang CS. Phototaxis of Haloarcula marismortui revealed through a novel microbial motion analysis algorithm. Photochem Photobiol. 2010; 86(5): 1084-90. (SCI)

2.         Fu HY, Lin YC, Chang YN, Tseng H, Huang CC, Liu KC, Huang CS, Su CW, Weng RR, Lee YY, Ng WV, Yang CS. A novel six-rhodopsin system in a single archaeon. J Bacteriol. 2010; 192(22): 5866-73. (SCI)

 

2009

1.         Chen TH, Huang YC, Yang CS, Yang CC, Wang AY, Sung HY. Insights into the catalytic properties of bamboo vacuolar invertase through mutational analysis of active site residues. Phytochemistry. 2009; 70(1): 25-31. (SCI)

 

2004

1.         Yang CS, Sineshchekov O, Spudich EN, Spudich JL. The cytoplasmic membrane-proximal domain of the HtrII transducer interacts with the E-F loop of photoactivated Natronomonas pharaonis sensory rhodopsin II. J Biol Chem. 2004; 279(41): 42970-6. (SCI)

 

2002

1.         Jung KH, Trivedi V, Yang CS, Sineschekov OA, Spudich EN, Spudich JL. Microbial Rhodopsins: Genome-mining, Diversity, and Structure/Function. J Photoscience. 2002; 9(3): 45-8. (SCI)

 

2001

1.         Yang CS, Spudich JL. Light-induced structural changes occur in the transmembrane helices of the Natronobacterium pharaonis HtrII transducer. Biochemistry-Us. 2001; 40(47): 14207-14. (SCI)

 

2000

1.         Yang CS, Skiba NP, Mazzoni MR, Thomas TO, Hamm HE. Fluorescent probes as indicators of conformation changes in transducin on activation. Methods Enzymol. 2000; 315: 490-502. (SCI)

2.         Spudich JL, Yang CS, Jung KH, Spudich EN. RETINYLIDENE PROTEINS: Structures and Functions from Archaea to Humans. Annu Rev Cell Dev Biol. 2000; 16(1): 365-92. (SCI)

 

1999

1.         Yang CS, Skiba NP, Mazzoni MR, Hamm HE. Conformational changes at the carboxyl terminus of Galpha occur during G protein activation. J Biol Chem. 1999; 274(4): 2379-85. (SCI)

2.         Skiba NP, Yang CS, Huang T, Bae H, Hamm HE. The alpha-helical domain of Galphat determines specific interaction with regulator of G protein signaling 9. J Biol Chem. 1999; 274(13): 8770-8. (SCI)

 

1998

1.         Ford CE, Skiba NP, Bae H, Daaka Y, Reuveny E, Shekter LR, Rosal R, Weng G, Yang CS, Iyengar R, Miller RJ, Jan LY, Lefkowitz RJ, Hamm HE. Molecular basis for interactions of G protein betagamma subunits with effectors. Science. 1998; 280(5367): 1271-4. (SCI)

 

Book

1.           Whi-Fin Wu, Chii-Shen Yang, Hsiang-Yun Lien, Fan-Ching Hsieh and Chung-Yang Chang (2008) HsuUV (ClpYQ), an ATP-dependent protease with unique characteristics. Research Signpost 37/661 (2), Fort P.O., Trivandrum-695 023, Kerala, India.

2.           Handbook of Photosensory Receptors (2005) Winslow R. Briggs and John L. Spudich. ISBN: 3-527-31019-3, Wiley, Hoboken, NJ, USA (內容貢獻及封面設計)

 

 

Last updated: 2013/09/26